Prediction of Key Genes and Pathways in the Pathogenesis of Vitiligo Based on Bioinformatic Analysis
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维普
万方数据
目的 通过生物信息学方法,寻找白癜风致病过程差异表达的基因,分析其功能,预测白癜风的可能发病机制.方法 从美国国家生物技术信息中心(NCBI)Gene database的获取GSE75819基因芯片数据集,以P＜0.05及| log FC |＞1.5筛选差异基因(DEGs),通过用于注释、可视化和集成发现的数据库(DAVID)官方平台,对差异基因进行人基因本体(GO)和京都基因和基因组数据库(KEGG)的生物功能富集分析,同时在基因/蛋白质互相作用检索分析工具(STRING)官方平台分析建立蛋白质相互作用(PPI)网络图,用Cytoscape软件分析有意义的集簇模块并对其进行基因功能和信号通路富集分析.结果 共筛选到13个上调基因和210个下调基因,对DEGs进行生物功能富集分析得到其生物途径、分子功能、细胞成分以及信号通路富集结果,从PPI网络图分析得到MRPL13、SNRPG等前10个核心基因及前7个有意义的模块,筛选第1个模块中RPL17、RPL34、RPL31等前10个枢纽基因,并富集分析出其基因功能及参与的信号通路.结论 MRPL13、SNRPG等基因可能参与白癜风的致病过程,RPL17等基因可能通过核糖体通路参与白癜风的发病机制.筛选核心基因、对其功能和作用通路富集分析为白癜风发病机制及治疗靶点的研究提供了理论依据.
Objective Through bioinformatic approach,we identify the differentially expressed genes in vitiligo,analysis their functions,and to predict the probable pathogenesis of vitiligo.Methods The GSE75819 gene chipset was obtained from the NCBI Gene database.Pick the differentially expressed genes(DEGs)limited for P＜0.05 and | log FC |＞1.5.The database for annotation,visualization and integrated discovery(DA VID)was used to perform Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses for DEGs.The Search Tool for the Retrieval of Interacting Genes(STRING)official platform was used to establish a protein-protein interaction(PPI)network.Cytoscape was used to identify meaningful cluster modules and then analyze the functions and pathway analysis of the core genes.Results A total of 13 up-regulated and 210 down-regulated genes were obtained in this study.GO and KEGG enrichment analysis were performed on the DEGs and showed the biological process,molecular function,cellular component and the pathway of the DEGs.Top 10 core genes such as MRPL13,SNRPG etc.and 7 meaningful clusters were selected from a PPI network.The 10 pivot genes obtained from the first cluster were RPL17,RPL34,RPL31,etc.Then their functions and pathways were analyzed.Conclusion Genes such as MRPL13,SNRPG may be involved in the pathogenic process of vitiligo,and RPL17 may be involved in the pathogenesis of vitiligo through the ribosome pathway.Discovering core genes and enrichment analyzing their functions and pathway in our study provide a theoretical basis for the study of vitiligo pathogenesis and therapeutic targets.
维普
万方数据
