摘要
目的 探究杉上斛多糖对特应性皮炎(AD)小鼠的干预效果以及对肠道菌群的影响作用.方法 将小鼠随机分为对照组(3只)、AD组(4只)和AD+杉上斛多糖组(4只),比较3组小鼠临床表现(搔抓次数、皮损评分、耳廓肿胀度),HE染色观察皮肤组织病理损伤.酶联免疫吸附测定(ELISA)法检测3组小鼠外周血和皮损部位免疫球蛋白E(IgE)、白细胞介素-4(IL-4)和干扰素-γ(IFN-γ)水平;定量聚合酶链反应(qPCR)检测3组小鼠IL-4和IFN-γmRNA水平.收集3组小鼠粪便进行16S rRNA测序分析.结果 与对照组比较,AD组小鼠出现红斑、水肿、表皮剥脱和鳞屑等病理表现,皮损评分显著升高;皮损部位角化过度伴角化不全,棘层肥厚,表皮层、真皮层可见明显的炎性细胞浸润,毛细血管扩张;血清及皮损部位的炎性因子表达水平显著升高;肠道菌群中拟杆菌属、葡萄球菌丰度增加,Muri菌、拟普雷沃氏菌属、毛螺菌科-NK4A136组丰度减少.使用杉上斛多糖干预后,AD小鼠的病理情况、皮损评分、皮损部位严重程度、炎性因子表达及差异菌群丰度均有改善.结论 杉上斛多糖通过调节肠道菌群平衡,减轻炎性反应以发挥药理作用,可有效缓解AD的皮损状况.
Abstract
Objective In order to explore the therapeutic efficacy of Dendrobium officinale polysaccharides on atopic dermatitis(AD)mice and its effects on gut microbes.Methods The mice were randomly divided into control group(three mice),AD group(four mice)and AD+Dendrobiumfugitivei polysaccharide group(four mice).Comparison of clinical manifestations(scratching times,skin lesion score,ear swelling)of the three groups of mice,HE staining was used to observe the pathological damage of skin tissue.The levels of IgE,interleukin-4(IL-4)and interferon-γ(IFN-γ)in the peripheral blood and skin lesions of the three groups of mice were examined by enzyme-linked immunosorbent assay(ELISA).The levels of IL-4 and IFN-γ mRNA in the three groups of mice were detected by quantitative polymerase chain reaction(qPCR).The mouse feces of three groups were collected for 16S rRNA gene sequencing.Results Compared with the control group,AD mice developed pathological manifestations such as erythema,edema,epidermal exfoliation and scaling,the skin lesion score was significantly increased;hyperkeratosis accompanied with hypokeratosis,hypertrophy of the spine,obvious inflammatory cell infiltration and telangiectasia were observed in the epidermis and dermis;the expression levels of inflammatory factors in serum and skin lesions were significantly increased;the abundance of Bacteroides and Staphylococcus increased in the intestinal flora,while the abundance of Muri,Prevotella,Lachnospiraceae-NK4A136-group decreased.After the intervention of Dendrobium officinale polysaccharides,the pathological condition,skin lesion score,skin lesion severity,inflammatory factor expression and differential flora abundance of AD mice were improved.Conclusion Dendrobium officinale polysaccharides can exert pharmacological effects by regulating the balance of gut microbiota and reducing inflammatory response,and can effectively relieve the skin lesions of AD.
基金项目
高新技术产业科技创新引领计划(2020SK2023)
河南省科技攻关计划(212102311120)
维普
万方数据