Objective In order to investigate the amelioration and anti-inflammatory effects of polygonum multiflorum extract(PME)on skin lesions of psoriasis mice,and to elucidate its possible mechanism.Methods Female BALB/c mice were randomly divided into normal group,model group,PME low-dose,medium-dose and high-dose groups,with 15 mice in each group.In addition to the normal group,imiquimod(IMQ)was used to induce psoriasis model for seven days.The skin lesions on the back of mice in each group were recorded,the lesion area and psoriasis severity index(PASI)score was performed.HE staining was performed on mouse dorsal epidermis.The positive expression of CD4 and K17 was detected by immunohistochemistry.The expression of tumor necrosis factor-α(TNF-α)and interleukin-8(IL-8)in skin tissues was detected by enzyme-linked immunosorbent assay(ELISA).The messenger RNA(mRNA)relative expression levels of IL-17A,IL-17F,IL-22 and IL-23 were detected by quantitative reverse transcription polymerase chain reaction(RT-qPCR).The relative expression levels of intercellular adhesion molecule-1(ICAM-1)and vascular cell adhesion molecule-1(VCAM-1)proteins were detected by Western blotting.Results Compared with normal group,PASI score of model group was increased(P<0.05).The thickening of the spinous layer was crista-like extension,there was obvious inflammatory edema,and the thickness of the skin epidermis increased(P<0.05).The positive expression of CD4 and K17,the content of IL-8 and TNF-α,the relative expression levels of IL-17A,IL-17F,IL-22 and IL-23 mRNA as well as the relative expression levels of ICAM-1 and VCAM-1 protein were significantly increased(P<0.05).Compared with model group,PASI score,inflammatory cell infiltration,spinous layer distortion and epidermal layer thickness were decreased in low,medium and high dose PME groups(P<0.05).CD4 and K17 positive expression,TNF-α and IL-8 content,IL-17A,IL-17F,IL-22,IL-23 mRNA relative expression level and ICAM-1,VCAM-1 protein relative expression level decreased(P<0.05).The effects of PME were dose-dependent.Conclusion PME could improve the skin lesions of IMQ-induced psoriasis mice,which may be related to the inhibition of IL-23/IL-17 inflammatory axis.
NETL
NSTL
万方数据
