Objective To investigate the role of intestinal fatty acid binding protein(I-FABP)and D-lactate(D-LAC)in the diagnosis of intestinal necrosis in incarcerated hernia.Methods Sprague Dawley rats were used for incarcerated hernia model,and then divided into control group(n=18)and experimental group(n=18).At 30 min,2 h,4 h,6 h,8 h and 12 h after operation,the levels of D-LAC and I-FABP in peripheral blood of the two groups were detected by ELISA.RT-qPCR was used to identify the expression of I-FABP in the intestinal tissue of incarcerated hernia.Intestinal necrosis was determined by gross specimen,HE staining and Chiu's score.Results Compared with the control group,the experimental group showed typical intestinal strangulation at 6 h after operation,Chiu's score was statistically significant(P=0.001),and the serum D-LAC level was significantly increased(P=0.002).At 8 h,the intestine gradually became necrotic,and the serum D-LAC level was further increased(P=0.012),the serum I-FABP level was also significantly increased(P=0.001),and the expression of I-FABP in intestinal tissue was significantly increased(P=0.002).At 12 h,the intestine showed obvious characteristics of intestinal necrosis,Chiu's score was statistically significant(P=0.001),and the serum D-LAC and I-FABP increased to the highest level[(2019.60±16.17)μg/L vs(273.18±14.63)μg/L,P=0.001;(1210.94± 5.96)p.g/L vs(220.46±9.63)μg/L,P=0.001].The expression of I-FABP in intestinal tissue was the highest[(8.20± 0.60)μg/L vs(1.13±0.16)μg/L,P=0.001].Conclusion The increased serum levels of I-FABP and D-LAC provide clinical evidence for the early diagnosis of intestinal necrosis in incarcerated hernia.
维普
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