中国中西医结合外科杂志2024，Vol.30Issue(4) ：486-492.DOI:10.3969/j.issn.1007-6948.2024.04.010

基于Wnt/β-catenin信号通路探讨清胰汤对重症急性胰腺炎肺泡上皮细胞损伤的修复机制

Based on Wnt/β-catenin signaling pathway to explore mechanism of Qingyi decoction in promoting repair of alveolar epithelial cell damage in severe acute pancreatitis

王茵 白卜月 陈海龙 闻庆平
中国中西医结合外科杂志2024，Vol.30Issue(4) ：486-492.DOI:10.3969/j.issn.1007-6948.2024.04.010
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基于Wnt/β-catenin信号通路探讨清胰汤对重症急性胰腺炎肺泡上皮细胞损伤的修复机制

Based on Wnt/β-catenin signaling pathway to explore mechanism of Qingyi decoction in promoting repair of alveolar epithelial cell damage in severe acute pancreatitis

王茵 1白卜月 2陈海龙 3闻庆平2
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作者信息

  • 1. 大连医科大学附属第一医院(大连 116000);西安交通大学第一附属医院麻醉手术部(西安 710061)
  • 2. 大连医科大学附属第一医院(大连 116000)
  • 3. 大连医科大学附属第一医院(大连 116000);大连医科大学中西医结合研究院(大连 116000)
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摘要

目的:探究Wnt/β-catenin信号通路在重症急性胰腺炎(SAP)肺损伤发病机制中的作用及清胰汤(QYD)的干预机制.方法:将40只大鼠分为假手术(Sham)组、Sham+QYD组、SAP组和SAP+QYD组.采用经胆胰管逆行注射3.5％牛磺胆酸钠建立SAP大鼠模型,造模48 h后取胰腺和肺组织,苏木素-伊红(HE)染色检测组织病理损伤,真空干燥法检测肺湿/干重比值(W/D),ELISA法检测血清白细胞介素(IL)-6水平,TUNEL试剂盒和蛋白免疫印迹法(WB)检测肺组织凋亡情况.免疫荧光法检测CyclinD1、β-catenin与SFTPC双染情况.WB法检测肺组织中Wnt/β-catenin信号通路关键蛋白和下游细胞周期蛋白的表达.结果:与Sham组相比,SAP组大鼠HE切片显示胰腺和肺部病变严重,IL-6含量明显升高,SFTPC表达减少,提示肺泡上皮细胞严重损伤.与SAP大鼠相比,清胰汤治疗后大鼠胰腺和肺组织病理评分、W/D等各项指标均有不同程度改善,IL-6含量减少,凋亡蛋白Bcl-2、Bax及Wnt/β-catenin信号通路关键蛋白和下游细胞周期蛋白表达明显上调,免疫荧光双染结果显示清胰汤治疗组出现更多的双染细胞,差异均有统计学意义(P＜0.05).结论:清胰汤通过激活Wnt/β-catenin信号通路减少肺泡上皮细胞凋亡,进而在促进急性胰腺炎相关肺损伤修复中发挥关键作用.

Abstract

Objective To explore the role of Wnt/β-catenin signaling pathway in the pathogenesis of lung injury in severe acute pancreatitis(SAP)and the intervention effect of Qingyi decoction(QYD).Methods 40 rats were divided into Sham group,sham operation group(Sham+QYD),SAP group,and QYD treatment group(SAP+QYD).The SAP model was established by retrograde injection of 3.5％sodium taurocholate through the biliopancreatic duct.After 48 hours of modeling,pancreatic and lung tissues were taken,and tissue pathological damage was detected by hematoxylin-eosin(HE)staining,the lung wet/dry weight ratio(W/D)was measured using vacuum drying method,serum IL-6 levels were detected using enzyme-linked immunosorbent assay(ELISA),and lung tissue apoptosis was detected using TUNEL assay and Western blot(WB).Immunofluorescence assay was used to detect double staining of CyclinD1 and SFTPC,β-catenin and SFTPC.WB method was used to detect the expression of key proteins in the Wnt/β-catenin signaling pathway and downstream cyclins.Results Compared with the Sham group,HE slices of SAP group rats showed severe pancreatic and pulmonary lesions,significantly increased serum IL-6 levels,and decreased SFTPC expression,indicating severe damage to alveolar epithelial cells.Compared with SAP rats,QYD treatment showed varying degrees of improvement in various indicators such as pancreatic and lung tissue pathological scores,W/D,IL-6 content,apoptotic proteins Bcl-2,Bax,and the expression of key proteins in the Wnt/β-catenin signaling pathway and downstream cyclins was significantly upregulated.The immunofluorescence double staining results showed that the QYD treatment group had more double stained cells,and the above differences were statistically significant(P＜0.05).Conclusion QYD through activate Wnt/β-catenin signaling pathway plays a crucial role in promoting the repair of acute pancreatitis related lung injury by reducing apoptosis of alveolar epithelial cells.

关键词

急性胰腺炎相关肺损伤/Ⅱ型肺泡上皮细胞/Wnt/β-catenin/清胰汤

Key words

Acute pancreatitis-associated lung injury/alveolar epithelial type Ⅱ cells/Wnt/β-catenin/Qingyi decoction

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基金项目

辽宁省重点实验室项目(LJKZ0851)

出版年

2024
中国中西医结合外科杂志
中国中西医结合学会 天津市中西医结合急腹症研究所

中国中西医结合外科杂志

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影响因子：0.918
ISSN：1007-6948
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