Objective To investigate the relationship between miR-28-5p expression and the prognosis of cholangiocarcinoma patients,as well as its functional role in tumor occurrence and development.Methods A total of 112 cholangiocarcinoma patients who underwent surgical resection were included in this study.The expression levels of miR-28-5p were detected in 112 pairs of cholangiocarcinoma tissues and adjacent non-cancerous tissues,as well as in CCA cell lines(TFK-1,RBE,HuH28,HuCCT-1,QBC939)and normal biliary epithelial cells(HIBEpic).The relationship between miR-28-5p and patient prognosis was analyzed using Kaplan-Meier curves and Cox regression analysis.The effects of miR-28-5p on cell proliferation,migration,and invasion were assessed using CCK-8 and Transwell assays.Results The expression levels of miR-28-5p in cholangiocarcinoma tissues and cell lines were significantly higher than those in adjacent non-cancerous tissues and normal epithelial cells.Additionally,low expression of miR-28-5p was significantly associated with poor prognosis in patients.Downregulation of miR-28-5p promoted proliferation,migration,and invasion of cholangiocarcinoma cells,while overexpression of miR-28-5p inhibited these processes.Conclusion miR-28-5p holds promise as a prognostic marker and potential therapeutic target for cholangiocarcinoma patients.
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