Objective To evaluate the effects and mechanism of Xiaojin pills for the treatment of a rat model of PD,and to research the level of TLR4-p38MAPK-MMP1 in the corpora cavernosa.Methods Twenty-four male Sprague-Dawley rats were randomly divided into three groups:control,PD and PD plus Xiaojin pills treatment.All rats underwent penile injections into the TA with 50 μL vehicle(control)or transforming growth factor(TGF)-β1(50 μg/rats)injected into the TA(remaining groups).The PD group was gavaged 50 μL water twice one day on the 42 days after TGF-β1 injection.The PD plus Xiaojin pills treatment group was gavaged 50 μL solution(50 μg Xiaojin pills)twice one day on the 42 days after TGF-β1 injection).Twenty-eight days following intragastricing,the penile tissues of all rats were harvested and stored at 80 ℃ for further analysis.Tissues were evaluated histologically and for expression of TLR4-p38MAPK-MMP1.Results Pathological HE staining showed that the PD group had more obvious fibrosis than the control group.The fibrosis of Xiaojin pill treatment group was significantly improved compared with PD group.The expression of TLR4-p38MAPK-MMP1 RNA in control group and Xiaojin pill treatment group was significantly lower than that in PD group(P＜0.01).The expression of TLR4-p38MAPK-MMP1 protein in control group and Xiaojin pill treatment group was significantly lower than that of PD group(P＜0.01).Conclusions There was obvious fibrosis and increased expression of TLR4-p38MAPK-MMP1 in penile sponges of rats with penile sclerosis.After Xiaojin pill treatment,the fibrosis was significantly improved and the expression of TLR4-p38MAPK-MMP1 was reduced.The treatment of penile sclerosis with Xiaojin pills may be related to the anti-fibrosis mechanism mediated by TLR4-p38MAPK-MMP1.
关键词
阴茎硬结症/小金丸/TLR4-p38MAPK-MMP1信号通路/大鼠/抗纤维化
Key words
Peyronie's disease/Xiaojin pills/TLR4-p38MAPK-MMP1 signal path/rat/antifibrosis
维普
万方数据