摘要
目的:探讨大肠主津理论在老年便秘模型大鼠中的作用机制.方法:使用复方地芬诺酯10 mg/kg灌胃持续2周,建立老年便秘大鼠模型.造模成功后将30只大鼠随机分为模型组、中药组及乳果糖组,每组10只,并设置空白对照组即正常组10只,各组用相应药物或生理盐水灌胃.给药2周后,测定各组大鼠粪便含水量、肠道炭末推进情况;苏木精-伊红(HE)染色进行组织学分析;实时荧光定量PCR检测结肠中AQP3、AQP4及AQP8 mRNA表达水平.结果:与正常组比较,模型组大鼠粪便含水率、炭末推进率明显降低;结肠组织肌层厚度减小、黏蛋白数量减少,肠屏障结构受到破坏;AQP3、AQP4及AQP8的mRNA表达均明显上调.与模型组比较,中药组与乳果糖组大鼠粪便含水率及炭末推进率明显升高;受到破坏的肠屏障结构明显恢复,结肠组织肌层厚度和黏蛋白数量增加;AQP3、AQP4及AQP8 mRNA表达均明显下调.中药组的大鼠粪便含水率、水通道蛋白表达均优于乳果糖组.结论:益肠通秘方在老年便秘模型大鼠中作用明确,其主要作用机制是能够快速恢复肠道组织病理形态学的损伤,通过调控结肠黏膜AQP3、AQP4、AQP8 mRNA水平提高粪便含水量,进而改善便秘.
Abstract
Objective To explore the mechanism of action of the theory of main body fluid of large intestine in aged constipation model rats.Methods The rat model of senile constipation was established by oral administration of compound difenoate 10 mg/kg for 2 weeks.The rats were randomly divided into model group,Chinese Medicine Group and lactulose group with 10 rats in each group,and the blank control group with 10 rats in each group.After 2 weeks,the fecal water content and intestinal carbon powder propulsive rate were measured,and Caesalpinia sappan eosin staining was used for histological analysis The mRNA levels of AQP3,AQP4 and AQP8 in colon were detected by real-time quantitative PCR.Results Showed that compared with the normal group,the water content of feces and the propulsive rate of activated carbon in the model group were significantly decreased,the thickness of colonic muscle layer was decreased,the amount of mucin was decreased,and the intestinal barrier structure was damaged The expressions of AQP3,AQP4 and AQP8 mRNA were up-regulated.Compared with the model group,the fecal water content and activated carbon propulsive rate of rats treated with Yichangtong were significantly increased,the damaged intestinal barrier structure was obviously recovered,the thickness of colonic muscle layer and the amount of mucin were increased The expressions of AQP3,AQP4 and AQP8 mRNA were down-regulated.The water content of the feces and the expression of aquaporin in the Chinese herbal medicine group were better than those in the lactulose group.Conclusion The effect of Yichangtong on senile constipation model rats is clear,and its main mechanism can quickly recover the damage of intestinal pathomorphology,and increase the water content of feces by regulating the levels of AQP3,AQP4 and AQP8 mRNA in colonic mucosa,improve constipation.
维普
万方数据