摘要
目的:通过观察通心络对链脲佐菌素诱导糖尿病大鼠心肌PCNA的影响,探讨通心络对糖尿病心肌病变的治疗价值及可能机制.方法:60只大鼠随机取17只作为对照组;将造模成功的40只糖尿病大鼠按随机数字表法分为模型8周、12周组和通心络治疗8周、12周组每组10只共4组,实验持续12周,每4周监测血糖1次,分别于8周、12周2个实验点取大鼠心室肌组织,免疫组化方法检测PCNA％.结果:模型组和通心络治疗组大鼠血糖水平均明显高于对照组(P＜0.05),模型8周、12周组心肌PCNA％较正常大鼠增加(P＜0.01),通心络治疗组心肌PCNA％较模型组明显减少(P＜0.01).结论:通心络可改善糖尿病大鼠心肌PCNA的表达,其对心肌病变的治疗机制可能与抑制心肌成纤维细胞PCNA的合成及表达有关.
Abstract
Objective:By observing the influence of Tong Xin Luo to diabetic cardiomyopathy rat PCNA that lead by STZ,and then to investigate therapeutic value and possible mechanism of Tong Xin Luo to diabetic cardiomyopathy.Method:60 four-week old masculinity depuratory SD rats weighted 210 ± 20 g.17 was selected as control group by random and raised normally without special treatment.The rest 43 SD rats were on absolute diet.After 12 hours,injected STZ of 1 mg/mL to create diabetic model.Then the 40 good ones was divided into 4 groups evenly at random:model week 8 group and week 12 group and Tong Xin Luo week 8 and week 12 therapy group.This experiment lasts for 12 weeks.Blood glucose monitored one time every 4 weeks.At week 8 and 12 about 10ml blood samples was taken from abdominal aorta and blood serum abstracted for later usage; ventricular muscle tissues was taken and fixed by formalin.Using immunity class masculine index to measure PCNA (photograph taken by computer image analytical system and percentage calculated).Result:Finally 53 rats went into results analysis.The level of blood glucose of exemplar groups and Tong Xin Luo groups were significantly higher than control groups (P ＜ 0.05).For control groups there were no significant difference between week 8 group and week 12 group in cardiac muscle PCNA expression(P ＞ 0.01).The expression of diabetes week 8 group and week 12 group increased compared with control groups and there was significant difference between the group (P ＜ 0.01) ; the expression of week 12 group increased more than week 8 group obviously and no significant difference in the group(P ＜0.01).The expression of cardiac muscle PCNA of Tong Xin Luo week 8 group and week 12 group decreased significantly than diabetes week 8 group and week 12 group(P ＜ 0.01).Conclusion:Tong Xin Luo could change the expression of cardiac muscle PCNA for diabetic cardiomyopathy rat.The therapy mechanism to diabetic cardiomyopathy may be due to the restraint of the composition and expression of cardiac myofibroblast PCNA.
国家科技期刊平台
NSTL
维普
万方数据