Discussion of Effect and Mechanism of Jianpi Shenshi Jiedu Decoction on Hyperuricemia Rats
Objective To observe the effect of Jianpi Shenshi Jiedu decoction on urate transporter expression in hyperuricemia(HUA)model rats,and explore the mechanism of Jianpi Shenshi Jiedu decoction in the treatment of hyperuricemia.Methods A total of 60 healthy male SD rats were randomly divided into control group,model group,benzbromarone group,and low,medium and high dose groups of Jianpi Shenshi Jiedu decoction.Except the control group was given distilled water,the other groups were intragastrically administered with potassium oxonate and adenine to establish a HUA rat model.One hour after intragastric administration of potassium oxonate and adenine,the rats were given corresponding drugs by gavage,then sacrificed after 21 days.Serum samples were collected to detect the content of serum uric acid(SUA),serum creatinine(SCR),blood urea nitrogen(BUN),aspartate aminotransferase(AST)and alanine aminotransferase(ALT)in rats.HE staining was used to observe morphological changes in kidney and liver tissues.The protein and mRNA levels of urate transporter(URAT)1 and organic anion transporter(OAT)3 were detected by Western blot and real-time qPCR.Results Compared with the control group,the levels of SUA and SCR,and the protein and mRNA level of URAT1 in the model group rats were significantly increased(P<0.05),while the protein and mRNA level of OAT3 was significantly decreased(P<0.01).Compared with the model group,the level of SUA in the Jianpi Shenshi Jiedu decoction low-dose group was significantly decreased(P<0.01),and the levels of SUA,SCR and BUN in Jianpi Shenshi Jiedu decoction medium-dose and high-dose groups were significantly decreased(P<0.05).In addition,high-dose Jianpi Shenshi Jiedu decoction could reduce the protein and mRNA level of URAT1(P<0.01),increase the protein and mRNA level of OAT3(P<0.05),and alleviate kidney injury.Conclusion Jianpi Shenshi Jiedu decoction has a significant uric acid lowering effect.Its mechanism may be related to the down-regulation of uric acid reabsorption protein URAT1 and the up-regulation of uric acid secretion protein OAT3 expression.
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