Effect of Yiqi Huoxue Formula on TLR4/MyD88/NF-κB Signal Pathway in Rats with Coronary Heart Disease Qi Deficiency and Blood Stasis Syndrome
Objective To investigate the effect of Yiqi Huoxue Formula(YQHXF)on rats with coronary heart disease with Qi deficiency and blood stasis syndrome.Methods 48 male SD rats were randomly divided into sham group,model group,YQHXF group and isosorbide mononitrate group.Rats in the latter 3 groups underwent surgery of coronary artery ligation.After 7 days,except for the sham operation group,the rats in the other groups were forced to swim for 14 days and their food intake was limited.At the same time,rats of YQHXF group and isosorbide mononitrate group were intervened with corresponding medicine for 28 days.Cardiac function of rats was detected by cardiac ultrasound.Blood stasis degree of rats was detected by whole blood viscosity.HE staining was used to detect the pathological changes of the heart tissue.Masson staining was used to detect the degree of myocardial fibrosis of rats.The mRNA and protein expression of toll-like receptor(TLR)4,myeloid differentiationfactor(MyD)88 and nuclear-factor(NF-κB)p65 in myocardium were detected by quantitative real-time PCR and immunohistochemistry.The content of interleukin(IL)-1β,IL-6 and TNF-α in serum of rats was detected by ELISA.Results Compared with sham group,rats in model group had decreased cardiac function(P<0.01),increased whole blood viscosity(P<0.01),disordered myocardial tissue arrangement,larger range of myocardial damage,and severe myocardial fibrosis(P<0.01),TLR4,MyD88,and NF-κB p65 mRNA and protein expression levels were increased in the myocardium(P<0.01),and the levels of IL-1β,IL-6,and TNF-α in the serum were increased(P<0.01).Compared with model group,after drug intervention,cardiac function of the rats was improved(P<0.05),whole blood viscosity was decreased(P<0.01),myocardial tissue was neat,damaged area of myocardial tissue was narrowed,myocardial fibrosis was relieved(P<0.01),the mRNA and protein expression level of TLR4,MyD88 and NF-κB p65 were lowered(P<0.05),the level of IL-1β,IL-6,TNF-α was decreased(P<0.05).Conclusion YQHXF can inhibit inflammation mediated by TLR4/MyD88/NF-κB p65 signaling pathways to play a therapeutic role in the rats with coronary heart disease with Qi deficiency and blood stasis syndrome.
Coronary heart diseaseQi deficiency and blood stasis syndromeYiqi Huoxue FormulaTLR4/MyD88/NF-κB signal pathwayInflammation
NETL
NSTL
万方数据
维普
