Effect of Icariin on DCs and CD4+T Cell Differentiation in Co-Culture with Heart Failure Serum
Objective To investigate the effect of icariin(ICA)on the differentiation of CD4+T cells into helper cell(Th)17 and regulatory cells(Treg)in co-cultures of Dahl salt-sensitive rat bone marrow-derived dendritic cells(DCs)and CD4+T cells in heart failure sera.Methods Fifty SPF-rated 5-week-old male Dahl salt-sensitive rats(model group)and 10 male Dahl salt-resistant rats of the same age(normal group)were fed to 18 weeks of age and then anesthetized to prepare heart failure serum and normal serum.DCs and CD4+T cells were collected from both rats,and DCs and CD4+T cells were co-cultured at a ratio of 1:10 after CD4+T cell purity was determined by flow cytometry.The co-cultured cells were divided into control group(normal serum),model group(heart failure serum),model group +vitamin D group(1×10-8 mol/L),and model group+icariin high、medium and low dose group.Cell specimens were collected 24 h after drug intervention.The proportion of positive expression of Th17 and Treg in DCs and CD4+T cells co-culture was detected by flow cytometry.ELISA was performed to detect interleukin(IL)-17,transforming growth factor(TGF)-β1,IL-6,IL-12 and IL-10 levels in the supernatant of DCs and CD4+T cells co-culture.Results The treatment results of ICA and vitamin D showed the same trend.Both significantly decreased Th17 cell positive expression rate and Th17/Treg ratio(P<0.01)and increased Treg positive expression rate(P<0.01),significantly increased IL-10 levels(P<0.01)and decreased IL-17,TGF-β1,IL-6 and IL-12 levels(P<0.05).Conclusion ICA and vitamin D can treat heart failure by regulating the balance of Th17/Treg in the body,decreasing the expression of pro-inflammatory cytokines IL-17,TGF-β1,IL-6,IL-12 and increasing the expression of anti-inflammatory cytokine IL-10.
Dendritic cellsCD4+T cellsHeart failureIcariinVitamin D
NETL
NSTL
万方数据
