Study on the Effect and Mechanism of Gegen Juju Fuling Formula on Hyperuricemia Rats
Objective To investigate the uric acid-lowering effect and possible mechanism of Gegen Juju Fuling formula by establishing a model of hyperuricemia(HUA).Methods A total of 42 male SD rats were randomly divided into normal group,model group,Gegen Juju Fuling formula low,medium,high dose group(6.15 g/kg,12.3 g/kg,24.6 g/kg),allopurinol group(0.03 g/kg),normal+Gegen Juju Fuling formula(normal administration group).Except for the normal group and the normal administration group,rats in other groups were intragastric with potassium oxazinate of 1.5 g/kg and adenine of 0.1 g/kg daily to establish HUA model for 21 consecutive days.Starting from the 8th day,except for the normal group and the model group,the rats in the other groups were intragastricated with Gegen Juju Fuling prescription and allopurinol every day for 14 days.The blood of the rats was collected after the modeling.Serum uric acid(SUA),blood urea nitrogen(BUN),serum creatinine(SCr),xanthine oxidase(XOD),adenosine deaminase(ADA),and alanine aminotransferase(ALT),aspartate aminotransferase(AST)were determined.The liver and bilateral kidneys of rats were taken,and the kidney coefficient was calculated.Pathological changes in the kidney and liver were observed by hematoxylin-eosin(HE)staining.Western blot was used to detect organic anion(OAT)1,OAT3,ATP-binding cassette sub-family G member(ABCG)2,recombinant urate transporter(URAT)1,glucose transporter type(GLUT)9 protein expression.Results Compared with the normal group,the kidney of the model group was larger,the surface was wrinkled and uneven,the color was lighter,the renal tubules were compensatory dilated,and urate crystals and vacuoles were visible.Body mass was significantly decreased(P<0.05),kidney mass was significantly increased(P<0.05),and kidney coefficient was significantly increased(P<0.05).The contents of serum SUA,BUN,SCr,XOD,and ADA were significantly increased(P<0.05).OAT1,OAT3,ABCG2 protein expressions decreased significantly(P<0.05),and URAT1,GLUT9 protein levels increased significantly(P<0.05).Compared with the model group,the rats in the treatment group showed significant improvement in the above indexes,and the difference was statistically significant(P<0.05).Conclusion Gegen Juju Fuling formula can improve the level of hyperuric acid in HUA rats by regulating the protein related to uric acid regulation.
Gegen Juju Fuling formulaHyperuricemiaKidneyGoutRat
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