Analysis of the Medication Law of Ye Tianshi in Treatment of Chest Bi and Heart Pain Based on Data Mining and Network Pharmacology
Objective Analysis of the medication law of Ye Tianshi in the treatment of chest Bi and heart pain and potential mechanisms of action of core drug combinations to provide a basis for clinical treatment.Methods The prescriptions for the treatment of chest Bi and heart pain disease from the Complete Book of Medicine of Ye Tianshi were selected,and a database was established to conduct statistical analysis of the frequency of Chinese medicine,association rule analysis,cluster analysis,and factor analysis with the help of Microsoft Excel 2021,SPSS Modeler 18.0 and SPSS 25.0.The BATMAN-TCM website,GeneCards,DrugBank,OMIM,and TTD databases were used to obtain the targets of the core drug combinations and diseases,and the STRING website and Cytoscape 3.9.1 software were used to analyze the target protein interactions and screen the core targets.Gene ontology(GO)and kyoto encyclopedia of genes and genomes(KEGG)enrichment analyses were performed on the Metascape website and the Bioinformatics website for core drug combinations with disease-related targets.Results The 100 recipes included in the list of criteria involved 169 Chinese herbal medicines,with the frequency of use of a single herbal medicine≥8 times as a high-frequency herbal medicine,including Fuling(45 times),Banxia(23 times),Guizhi(23 times),and Shengjiang(21 times),etc.The medicinal properties were mostly warm and moderate,and the taste of the medicines was mostly sweet,bitter,and pungent,and they mostly belonged to the heart,lung and spleen meridians.The top three effects are to tonify deficiency,to resolve phlegm and cough,and to induce dampness.The association rule analysis yielded 11 groups of strongly associated drug combinations,with the highest confidence level being Banxia-Shengjiang-Fuling.Cluster analysis yielded five drug combinations and three new prescriptions,and factor analysis yielded eight male factors and three new prescriptions,most of which were effective in warming Yang,dispelling phlegm,invigorating Blood,tonifying Blood,benefiting Qi,and nourishing Yin.A network pharmacology study has identified 205 targets associated with the core drug pair Banxia-Shengjiang-Fuling for the treatment of chest Bi and heart pain,with tumor necrosis factor(TNF),fos proto-oncogene(FOS),nitric oxide synthase 3(NOS3)as key targets,and calcium(Ca2+)signaling pathway and cyclic guanosine monophosphate-protein kinase G(cGMP-PKG)signaling pathway as potential signaling pathways for the treatment of chest pain.Conclusion Ye Tianshi mainly selected prescription drugs from the three aspects of warming Yang and removing phlegm,promoting blood circulation and removing blood stasis,and invigorating Qi and nourishing Yin.The core medicine pair of Banxia-Shengjiang-Fuling may regulate calcium signaling pathway,cGMP-PKG signaling pathway through TNF,FOS,NOS3 and other targets,to play a role in improving chest Bi and heart pain.
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