Functional Mechanism of Xijiao Dihuang Decoction in Treating Psoriasis Based on Bioinformatics
Objective To screen out the characteristic genes related to psoriasis inflammation and explore the intervention mechanism of Xijiao Dihuang decoction with Bioinformatics.Methods Screened the dataset including psoriasis skin lesions and normal controls from the GEO database,and obtained the gene related to psoriasis inflammation from the GSEA database.Limma was used to screen differentially expressed genes,which were intersected with inflammation-related genes to obtain differentially expressed inflammation-related genes(DEIRGs).GO and KEGG enrichment analysis were carried out for them.WGCNA and LASSO were used to screen out the characteristic genes related to psoriasis inflammation,and an ROC test was performed.Searched TCMSP and TCMID databases,and screened the active ingredients and targets of Xijiao Dihuang decoction,and intersected with DEIRGs to obtain effective ingredients and targets.Then,the related structure files were obtained through Pubchem and PDB databases,the molecular docking was obtained and the binding energy was calculated by Swissdock database.Results In this study,560 differentially expressed genes and 20 DEIRGs were screened.GO and KEGG enrichment analysis revealed that 779 biological functions and 37 pathways were enriched.A total of 483 genes were screened by WGCNA,and 9 core psoriasis inflammation genes were obtained by intersection with 20 DEIRGs.6 inflammatory characteristic genes highly related to psoriasis were screened by LASSO,namely CXCL10,F3,NMI,P2RY2,RTP4,and TPBG.The ROC curve showed that the AUC of each gene was greater than 0.9,and the AUC of LASSO was 1.The traditional Chinese medicine ingredients in Xijiao Dihuang decoction were searched and analyzed through network pharmacology.The results showed that the active component ellagic acid in Paeoniae Radix Rubra can act on CXCL8 and the active component paeoniflorin can act on IL-6.Additionally,the active component quercetin in the Moutan Cortex can act on CXCL8,IL-6,F3,and CXCL10.Conclusion CXCL10,F3,NMI,P2RY2,RTP4,and TPBG might be the characteristic genes related to psoriasis inflammation.Paeoniae Radix Rubra and Moutan in Xijiao Dihuang decoction were predicted to act on the characteristic genes related to psoriasis,which could provide a theoretical reference for future drug research.
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