Study on the Effect and Mechanism of Sishen Pill on Irritable bowel syndrome Combined with Depression Model Rats
Objective To explore the effect of Sishen Pill on Irritable bowel syndrome combined with depression model rats.Methods SD male rats were randomly divided into normal group,model group,fluoxetine hydrochloride group and Sishen pill low-dose,medium-dose and high-dose groups.The model was established by giving sennae folium decoction combined with chronic unpredictable mild stress.Then the abdominal withdrawal reflex,fecal water content determination and sugar water preference test were used to verify the model.The drug was given for 14 days after the model was copied successfully.After administration,intestinal sensitivity and fecal water content of rats were detected,sugar water preference test,open field test and forced swimming test were performed.Pathological changes of hippocampus and colon were observed by HE staining.The levels of 5-hydroxytryptamine(5-HT),dopamine(DA),norepinephrine(NE),brain-derived neurotrophic factor(BDNF)in hippocampus,5-HT in blood and colon were measured by ELISA.Results Compared with the normal group,the morphological structure of hippocampus and colon in model group was damaged with inflammatory cell infiltration,and the preference rate of sugar water and locomotor activity were significantly decreased(P<0.01),while the intestinal sensitivity,fecal water content and immobility time of forced swimming were significantly increased(P<0.01),and the contents of 5-HT,DA,NE and BDNF in hippocampus were significantly decreased(P<0.01),while 5-HT contents in blood and colon increased(P<0.01).Compared with the model group rats,all the above indexes were improved to some extent in the rats in each dose group of Sishen pill and fluoxetine hydrochloride group.Morphological structural damage of hippocampus and colon in high dose group of Sishen pill was repaired,no inflammatory cell infiltration was observed.The preference rate of sugar water and locomotor activity were significantly increased(P<0.05),the intestinal sensitivity,fecal water content and immobility time of forced swimming were significantly decreased(P<0.01)in the Sishen pill high-dose groups.The contents of 5-HT,DA,NE and BDNF in hippocampus increased significantly(P<0.01),while the content of 5-HT in blood and colon decreased significantly(P<0.01)in the Sishen pill high-dose groups.Conclusion Sishen pill can improve the intestinal symptoms and depressive-like behaviors in irritable bowel syndrome combined with depression model rats,which may be related to the regulation of hippocampal neurotransmitters,serum and colon 5-HT levels,and the recovery of intestinal and central nervous system functions.
NETL
NSTL
万方数据
维普
