Study on the Differential Effects of Guizhi Decoction-Containing Serum Prepared from Two Different Blood Collection Sites on Its Anti-inflammatory Action
Objective To investigate the differences in anti-inflammatory effects between drug-containing serum prepared from two different blood collection sites using Guizhi decoction.Methods Male SD rats were administered Guizhi decoction at a dosage of 1 g/mL,ten times the clinical dose.Drug-containing serum was collected from both the portal vein and peripheral blood.RRLC-QQQ-MS/MS method was developed to quantify eight chemical constituents in both the Guizhi decoction and the drug-containing serum.Mouse brain-derived endothelial cells(bEnd.3)in the logarithmic growth phase were utilized to establish an inflammation model stimulated by IL-1β,with bEnd.3 cells also serving as the control group.Apart from the control and model groups,there were high,medium,and low dosage groups for Guizhi decoction(5%,2.5%,and 1.25%of the decoction respectively),as well as high,medium,and low dosage groups for drug-containing serum from the portal vein and peripheral blood(20%,10%,and 5%of serum respectively).The levels of PGE2 in the extracellular fluid were measured by ELISA.Results The analysis revealed that the decoction had relatively high concentrations of paeoniflorin,paeonol,glycyrrhizin,and glycyrrhetinic acid,while the serum was rich in cinnamic acid and 6-gingerol,along with new metabolites like glycyrrhetinic acid monoglucuronide.The content of these compounds was higher in the portal vein drug-containing serum compared to the peripheral blood serum.The concentration of glycyrrhetinic acid in 5%Guizhi decoction was 798.13 ng/mL,in 20%portal vein drug-containing serum was 316.22 ng/mL,and in 20%peripheral blood serum was 481.17 ng/mL,indicating similar levels of constituents in the drug across these mediums.When compared with the model group,the PGE2 levels in the extracellular fluid of bEnd.3 cells treated with varying doses of Guizhi decoction were significantly higher(P<0.05),whereas those in groups treated with drug-containing serum from both the portal vein and peripheral blood were significantly lower(P<0.05).Furthermore,the portal vein serum exhibited a markedly stronger pharmacological effect than the peripheral blood serum at equivalent concentrations(P<0.05).Conclusion Post-oral administration of Guizhi decoction,the drug-containing serum from the portal vein exhibited higher constituent levels and more potent anti-inflammatory effects compared to the peripheral blood serum.This finding highlights the significance of using portal vein drug-containing serum over traditional peripheral blood serum in research on Chinese herbal formulas.
Guizhi decoctionDrug-containing serum of portal vein bloodDrug-containing serum of peripheral bloodContent determinationPharmacodynamic experiment
NETL
NSTL
万方数据
维普
