Exploring the Mechanism of Qizhu Zengli Fang in Treating Cancer-Related Fatigue Based on Network Pharmacology and Nude Mouse Experiments
Objective By observing the effects of Qizhu Zengli Fang on cancer-related fatigue(CRF)in nude mice,to explore its therapeutic effects.Methods The active ingredients of Qizhu Zengli Fang were searched on the TCMSP platform,and converted into relevant targets through the Uniprot database.The targets related to CRF were searched in the GeneCards database,OMIM database,and DrugBank database.Then,the Jvenn platform was used to draw the intersection map of drugs and disease targets.The String platform was used to create a protein-protein interaction networks(PPI),and the Metascape platform was used to create GO and KEGG enrichment analysis.Among them,selected the proteoglycans in cancer signaling pathway for research.25 nude mice were randomly divided into blank group,model group,Chinese medicine group,chemotherapy group(cisplatin)and dexamethasone group.The CRF model was constructed.After 3 weeks of intervention,GPC1,GPC5,SDC2,and SRGN were detected by ELISA.Observed the pathological morphology of tumor tissue in nude mice in each group under light microscope.Results Compared with model group,the expression of GPC1 in the Chinese medicine group and the chemotherapy group was significantly decreased(P<0.01).The expression of SDC2 were decreased in Chinese medicine group(P<0.05).The SRGN of the Chinese medicine group,chemotherapy group,and dexamethasone group decreased(P<0.05).Conclusion Qizhu Zengli Fang can relieve fatigue by decreased the expression of GPC1,SDC2,and SRGN in tumors of nude mice,and its mechanism was speculated to be related to the inhibition of tumor growth and metastasis.
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