Comparative Research on the Protective Effect of Wuling San and Mahuang Wuling San on Renal Function in Rats with Adriamycin Induced Nephropathy
Objective To observe and compare the intervention effects of Wuling San and Mahuang Wuling San on adriamycin nephropathy(AN)rats and study their mechanisms of action.Methods Fifty SD rats were randomly divided into five groups:control group,model group,benazepril group,Wuling San group,and Mahuang Wuling San group.Except for the control group,the rat model of nephropathy was established by tail vein injection of adriamycin hydrochloride.Each treatment group was intragastrically administered with the corresponding drugs,the body weight of rats was monitored weekly,and 24-hour urine volume and urine protein were measured after five weeks,SCr,SUA,and BUN levels were measured,the expression levels of nephrin and podocin were detected by Western blot,and pathological changes in renal tissues were observed by HE staining.Results Compared with the control group,the model group showed a significant decrease in body weight(P<0.01),reduced urine volume(P<0.05),significantly increased 24-hour urine protein(P<0.01),and significantly increased levels of SCr,SUA,and BUN(P<0.01).HE staining showed irregular cell morphology,deformed nuclei,chromatin marginalization,and significantly suppressed expression levels of nephrin and podocin proteins(P<0.01).Compared with the model group,the body weight and urine volume increased(P<0.05),the 24-hour urinary protein decreased significantly(P<0.05),the SUA and BUN levels decreased significantly(P<0.01),the microscopic pathological damage of rat kidney tissue was significantly alleviated,and the expression of nephrin and podocin increased significantly in the Mahuang Wuling San group(P<0.01).Compared with the Wuling San group,the Mahuang Wuling San group showed a significant increase in urine volume(P<0.01),a significant decrease in 24-hour urine protein(P<0.05),and a significant increase in nephrin protein expression(P<0.01).Conclusion Mahuang Wuling San can effectively inhibit renal tissue injury,reduce urinary protein,improve renal function in rats with AN.Compared with Wuling San,Mahuang Wuling San has more pronounced diuretic and proteinuria-inhibiting effects.