Exploration of the Anti Epileptic Effect of Pingxian Granules from the Perspective of Blood-Brain Barrier Drug Transport Mechanism
Objective Observe the anti-epileptic effect of Pingxian granule and explore its mechanism.Methods 62 Wistar rats aged 3 weeks were randomly divided into two groups:a control group with 12 rats and a model group with 50 rats.The Pentetrazol(PTZ)intraperitoneal injection was used to induce the epilepsy model.After the successful preparation,the model rats were randomly divided into a model group,a western medicine group,and a Pingxian granule group,with 12 rats in each group.The western medicine group was administered with sodium valproate by gavage,the Pingxian granule group was administered with Pingxian granule by gavage.After 7 days of intervention,the morphological structure of the motor cortex in epileptic rats was observed.Immunohistochemistry,RT-qPCR,and Western blot were used to detect the expression of L-type amino acid transporter(LAT)1,P-glycoprotein(P-gp),and breast cancer resistance protein(BCRP)in the motor cortex tissue of the brain.Results The number of neurons in the motor cortex,and the expression of LAT1 protein and mRNA decreased(P＜0.05),while the expression of P-gp,BCRP and their mRNA increased(P＜0.05)for the model group rats,compared with the control group.Furthermore,the number of neurons in the motor cortex of rats in the Pingxian granule group increased(P＜0.05),and the pathological morphology of neurons was improved.LAT1 and its mRNA expression were upregulated(P＜0.01),while P-gp,BCRP,and their mRNA expression were downregulated(P＜0.01),compared with the model group.Conclusion Pingxian granule may exert its anti-epileptic effect by regulating the expression of blood-brain barrier(BBB)drug transporters P-gp,BCRP and LAT1.
EpilepsyPingxian granulesBlood-brain barrierDrug transport mechanismL-type amino acid transporter 1P-glycoproteinBreast cancer resistance protein
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