TMP Improves Oxidative Stress and Inflammatory Injury After Transient Global Brain Ischemia by Inhibition of NADPH Oxidase and Myeloperoxidase in Mice
Objective To investigate the effects of TMP on oxidative stress injury and NADPH oxidase expression in transient global brain ischemia mice.Methods The model of middle cerebral artery occlusion/reperfusion was established by means of thread embolism.Longa nerve function was evaluated after modeling.HE and triphenyltetrazolium chloride staining(TTC)were used to observe the pathological changes and infarction of brain tissue.The changes of total antioxidative capacity(T-AOC),superoxide dismutase(SOD),malondialdehyde(MDA),hydrogen peroxide(H2O2),and myeloperoxidase(MPO)in brain tissue were detected.The expression of NADPH oxidase activated P47phox,NADPH Nox4 proteins and TNF-α were detected by Western blot.Results TMP can significantly reduce the brain tissue injury of cerebral ischemia-reperfusion mice,reduce the volume of cerebral infarction,increase the levels of T-AOC and SOD,and decrease the contents of MDA,H2O2 and MPO(P<0.05).Meanwhile,TMP can reduce the expression of P47phox,Nox4 and TNF-α in cerebral tissue(P<0.05).Conclusion TMP has obvious protective effect on brain injury in cerebral ischemia-reperfusion mice,and the mechanism is related to reducing the expression of NADPH oxidase,inhibiting oxidative stress and alleviating inflammation.