Objective To replicate the dynamic evolution process of chest obstruction heart blood stasis syndrome,and to provide a more systematic evaluation system for the animal model of chest obstruction heart blood stasis syndrome. Methods 36 healthy SD male rats were randomly divided into normal and model groups. The model group was fed with high fat plus vitamin D3 by gavage and isoproterenol injection to construct a dynamic evolution model of chest obstruction heart blood stasis syndrome. Tongue,blood lipid,electrocardiogram,cardiac ultrasound,blood rheology and other indicators were observed. Results Tongue R values decreased in the sub-blood stasis syndrome stage compared with the pre-blood stasis syndrome stage (P<0.05),tongue R,G values decreased significantly in the heart blood stasis syndrome stage compared with the sub-blood stasis syndrome stage (P<0.05). Plasma total cholesterol and low-density lipoproteins increased significantly in the pre-blood stasis rats compared with the normal group (P<0.05),high-density lipoproteins increased significantly in the sub-blood stasis rats compared with the Pre-blood stasis rats (P<0.05),total plasma cholesterol,high-and low-density lipoproteins were significantly higher in rats with heart blood stasis syndrome than in the normal group (P<0.05). Low,medium,and high shear rates of whole-blood viscosity were significantly higher in rats with pre-blood stasis and heart blood stasis syndrome than in the normal group (P<0.05). The electrocardiogram S-T of rats with heart blood stasis syndrome elevated more than 0. 1 mV,and the left ventricular end-systolic internal diameter,left ventricular end-systolic volume index were significantly higher(P<0.05),while ejection fraction was significantly lower (P<0.05) than that of the normal group. The pathological results of aorta showed that the intima-media integrity was partially destroyed in rats in the pre-blood stasis syndrome. The intima-media of rats in the sub-blood stasis syndrome was unevenly thick and thin,the integrity of the outer membrane was destroyed,and the elastic fibers of the middle membrane were disorganized. As well as the intima-media of rats in the stage of heart blood stasis syndrome was markedly thickened and protruded,with the existence of a cavity under the intima-media,cholesterol crystals inside,cellular accumulation,disorganized elastic fibers of the middle membrane,and damaged integrity of the outer membrane. Conclusion Through the establishment of the dynamic model of chest obstruction heart blood stasis syndrome,we scientifically and comprehensively evaluated the serious stagnation of phlegm,turbidity and stasis in the dynamic evolution of chest obstruction heart blood stasis syndrome. It can provide a reference for the construction of a more systematic and perfect evaluation system for the animal model of chest obstruction heart blood stasis syndrome.
维普
万方数据
