调脾增食颗粒对幼龄厌食大鼠血浆β-EP及CCK-8的影响
Effects of Tiaopi Zengshi Granule(调脾增食颗粒)on Plasma β-EP and CCK-8 Levels in Juvenile Anorexia Rats
梁雪 1李会影 1于映霞1
作者信息
- 1. 吉林省中医药科学院·吉林 长春 130021
- 折叠
摘要
目的:探讨调脾增食颗粒对幼龄厌食大鼠血浆β-EP(β-内腓肽)及CCK-8(胆囊收缩素)的影响.方法:选取60 只幼龄SD大鼠,随机分为6 组,正常对照组,模型对照组,调脾增食颗粒高、中、低剂量组及阳性对照组,每组10 只.除正常对照组喂饲常规鼠饲料外,其余各组均采用特制高蛋白高脂饲料喂养1 周,建立幼龄厌食大鼠模型.正常对照组和模型对照组给予蒸馏水,调脾增食颗粒高、中、低剂量组按33.86、16.93、8.47 g/(kg·d)剂量给药,阳性组给予健胃消食片 0.675 g/(kg·d),连续给药 4 周;记录各组大鼠进食量,应用放射免疫法测定大鼠血浆β-EP及CCK-8 的含量.结果:调脾增食颗粒中、高剂量组第3 周和第4 周的进食量明显多于模型对照组(P<0.05,P<0.01).调脾增食颗粒中、高剂量组大鼠血浆β-EP含量明显高于模型组(P<0.05,P<0.01),CCK-8 含量明显低于模型组(P<0.05,P<0.01).结论:调脾增食颗粒治疗厌食症与调节血β-EP及CCK-8 的分泌与释放有关.
Abstract
Objective:To investigate the effects of Tiaopi Zengshi Granule on plasma β-EP and CCK-8 levels of juvenile anorexia rats.Methods:60 young SD rats were randomly divided into 6 groups:normal control group,model control group,Tiaopi Zengshi Granule high,middle and low dose groups and positive control group with 10 rats in each group.Except the normal control group,all the other groups were fed with special high-protein and high-fat diet for one week to establish the models of anorexia.The normal group and the model group were given distilled water,and the high,middle and low dose groups of Tiaopi Zengshi Granule were given Tiaopi Zengshi Granule 33.86,16.93,8.47 g/(kg·d),positive group was given Jianwei Xiaoshi Tablet 0.675 g/(kg·d),all were intragastric administration for 4 weeks,food consumption of each group rats were recorded,the contents of plasma β-EP and CCK-8 in rat were determined by radioimmunoassay.Results:The food con-sumption of Tiaopi Zengshi Granule middle and high dose groups at the 3rd and 4th week was significantly higher than that of model control group(P<0.05,P<0.01).The contents of plasma β-EP in Tiaopi Zengshi Gra-nule middle and high dose groups were significantly higher than that in model control group(P<0.05,P<0.01),The content of plasma CCK-8 in Tiaopi Zengshi Granule middle and high dose groups was significantly lower than those in model control group(P<0.05,P<0.01).Conclusion:Tiaopi Zengshi Granule can regu-late the secretion and release of plasma β-EP and CCK-8 in anorexia rats.
关键词
调脾增食颗粒/厌食/进食量/β-EP/CCK-8/幼龄大鼠Key words
Tiaopi Zengshi Granule/anorexia/food consumption/β-EP/CCK-8/juvenile rat引用本文复制引用
基金项目
吉林省卫生健康科技能力提升项目(2022JC011)
出版年
2024
国家科技期刊平台
NSTL
万方数据