Study on Improvement Effects of Phenylethanoid Glycosides Extract from Rouchongrong(Cistanche deserticola)on Lipid Metabolism in Livers of ApoE-/-Mice with High Fat Diet Based on Regulating ACOX1-CPT2 Expressions
Objective:To explore the effects of Phenylethanoid glycosides extract from Cistanche deserticola(PGCD)on lipids metobolism in ApoE-/-mice liver which induced by high fat diet.Methods:70 male ApoE-/-mice fed with high-fat diet were divided into 5 groups,model(MOD)group,positive control group mice were given Atorvastatin[20 mg/(kg·d)]+ Ezetimibe[20 mg/(kg·d)](A +E),PGCD low,middle and high dose groups were given PGCD[250,500,1000 mg/(kg·d)];another 14 male C57BL/6J mice with a chow diet were set as the normal control(CON)group.At the 12th weekend,the content of TG and the activities of ALT,AST in serum and liver tissue were detected according to the operating instructions.Fresh liver tissue was homogenized and the contents of fatty acid synthase(FAS),long-chain acyl-CoA dehydrogenase(LCAD)and glutathione peroxidase(GSH-Px),superoxide dismutase(SOD)activities were tested by ELISA method,meanwhile,the contents of malondialdehydeand(MDA)and lipid peroxide(LPO)were detected with kid methods.The mRNA expressions of acyl coenzyme A oxi-dase 1(ACOX1)and carnitine palmitoyltransferase 2(CPT2)were detected by RT-PCR experiments.Results:In contrast to the model group,three-dose PGCD were all reduced the levels of TG in liver and serum with a dose-dependent manner,and lowered the activities of ALT and AST in serum.Three-dose PGCD were all reduced the content of FAS with a dose-dependent fash-ion,and raised the content of LCAD in liver significantly.PGCD promoted the activities of GSH-Px and SOD and reduced the contents of MDA and LPO in liver significantly.RT-PCR experiments showed that PGCD ele-vated the mRNA levels of ACOX1and CPT2 in liver tissue.Conclusion:PGCD can ameliorate lipid metabolism and oxidative stress response,reduce lipid deposition in liver tissue by regulating the mRNA expressions of ACOX1 and CPT2.
Phenylethanoid glycosides extract from Cistanche deserticolaACOX1CPT2lipid metabolismoxidative stressApoE-/-mice
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