Antipyretic Effects of Baicalin and Effects on Serum TNF-α,IL-1β,IL-6,PGE2,cAMP and Cerebral NF-κB Expression in Dry Yeast Induced Febrile Rats
Objective:To study the antipyretic effect of Baicalin on dry yeast induced fever in rats and ex-plore its mechanism.Methods:The rat fever model was established by subcutaneous injection of dry yeast on the back.Male SD rats were randomly divided into normal control group,model group,positive group(Aspirin,0.1 g/kg),Baicalin high-dose,medium-dose and low-dose groups(160,80,40 mg/kg)for 3 days con-secutively.The changes of anal temperature in each group rats were determined after modeling.Serum TNF-α,IL-1β,IL-6,PGE2 and cAMP were measured by ELISA.Western Blot was used to detect the expression of NF-κB p65 protein in the brain of each group.Results:Baicalin high-dose group had a significant antipyretic effect(P<0.01),and all Baicalin groups reduced the contents of TNF-α,IL-1β,IL-6,PGE2 and cAMP in serum of rats to varying degrees.Compared with normal group,the expression of NF-κB p65protein in brain tissue of model group was increased,and the expression of NF-κB p65 in brain tissue of in Baicalin high-dose group was significantly decreased(P<0.05).Conclusions:Baicalin could significantly reduce the tempera-ture increase caused by dry yeast.The antipyretic mechanism may be related to reducing the serum TNF-α,IL-1β,IL-6,PGE2,cAMP levels and cerebral NF-κB p65 protein expression.
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