Effects of Sequential Method of Invigorating Kidney and Activating Blood on Endometrial Morphology and Expressions of VEGF,Vimentin and CK 12 Protein of Rats with Thin Endometrium
Objective:To observe the effects of the sequential method of invigorating kidney and activating blood on the morphology of endometrium and the expressions of endometrial VEGF,vimentin and CK 12 protein of rats with thin endometrium.Methods:50 female SD rats of SPF grade were randomly divided into model group,low-dose group,medium dose group,high-dose group,Progynova group(positive group)after 8 days of continuous administration of hydroxyurea with each 10 rats and 10 rats were taken as sham operation group.The sham operation group and model group were gavaged with 10 mL/kg distilled water,and the Progynova group was gavaged with 0.3 mg/kg Progynova,the three dose of Chinese medicine groups were given low,medi-um and high doses of Xiaozheng Mixture by gavage during estrus,low,medium and high doses of Yangmo Zhuyun Mixture were given by gavage in the proestrus stage,estrus stage and metestrus stage,respectively,for 3 estrus cycles,a total of 15 days.After the intervention,HE staining was used to observe the morphology of rats'endo-metrium;The expressions of vascular endothelial growth factor(VEGF),vimentin and cytokeratin 12(CK12)were detected by immunohistochemistry.Results:The endometrial thickness,the number of glands and blood vessels in the model group were significantly lower than those in the normal group(P<0.01).The endometrial thickness,the number of glands and blood vessels in the medium and high dose groups and Progynova groups were significantly higher than those in the model group(P<0.05 or 0.01).The expression of VEGF in the en-dometrium of the medium and high dose groups and the Progynova group was significantly higher than that of the model group(P<0.01).The expression of vimentin protein in endometrium of high dose group was significantly higher than that of model group(P<0.01).The expression of CK12 in endometrium of low,medium and high dose groups and Progynova group was significantly larger than that of model group(P<0.05 or 0.01).Conclu-sion:The sequential method of invigorating kidney and activating blood can increase the endometrial thickness and the expressions of VEGF,vimentin and CK12 protein in the endometrium of thin endometrium model rats.
sequential method of invigorating kidney and activating bloodthin endometriumXiaozheng Mix-tureYangmo Zhuyun MixtureProgynovaVEGFVimentinCKrat
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