Effects of Astragalus Polysaccharide on Inhibitory Ability of PD-1 Inhibitor in H22 Tumor-Bearing Mice
Objective:To observe the effects of APS on anti-cancer ability of PD-1 inhibitors on H22 tumor-bearing mice model and explore the possible mechanism.Methods:Hepatocellular carcinoma H22 cells were inoculated subcutaneously into BALB/c mice.H22 tumor-bearing mice were randomly divided into differ-ent group which treated with PD-1 inhibitor and/or different concentrations of APS.Tumor size was recorded,serum cytokines levels such as TNF-α,IL-1β,IL-2,IFN-γ,IL-4,IL-10,TGF-βwere determined by ELISA,PD-1 expression in tumor and spleen tissue was determined by Western Blot,tumor-infiltrating cell types were determined by flow cytometry.Results:1)Both APS and PD-1 inhibitors could inhibit tumor growth in the H22 tumor-bearing mice,and the effect of combination therapy was more significance and the inhibitory intensity increasedwiththeincreasingof APSconcentration.2)Theserumconcentrationsof TNF-α,IL-1β,IFNγ,IL-10 and TGF-β were increased after drug intervention,the serum concentrations of IL-2 and IL-4 were decreased,the expression of PD-1 protein in tumor and spleen tissue was decreased,and the infiltration ratio of CD+4 CD-8 cells and CD+4 CD+8 cells in tumor were increased.Conclusion:APS can enhance the inhibitory ability of PD-1 inhibitor on H22 hepatocellular carcinoma,which may be related to regulating cytokines secre-tion,inhibiting PD-1 expression,and increasing lymphocyte infiltration in tumor tissue.
Astragalus polysaccharideimmune checkpointhepatocellular carcinomagrowth of carcinomacytokinePD-1 proteinmouse
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