Exploring on Mechanism of Jiangweicao(Micromeria Biflora)in Reducing Proteinuria of Doxorubicin-Induced Nephropathy Rats based on VEGF Change
Objective:To observe the effect of Micromeria biflora on proteinuria in rats with doxorubicin-induced nephropathy and explore its mechanism.Methods:40 rats were divided into normal control group,model group,Chinese medicine group and Western medicine group.Injecting doxorubicin on tail vein one-time to establish doxorubicin nephropathy rat models.Two weeks after modeling,the normal control group and the model group were given normal saline by gavage,and the Chinese medicine group and the Western medicine group were given Micromeria biflora 900 mg/(kg·d)and Benazepril hydrochloride 0.9 mg/(kg·d)by gavage respectively for 4 weeks.The 24 h urinary protein(24 h TP),blood urea nitrogen(BUN),serum creatinine(SCr),albumin(ALB),the vascular endothelial growth factor(VEGF)content in plasma and kidney tissues were detected.Results:The 24 h TP of model group,Chinese medicine group and Western medicine group was significantly higher than that of normal control group.After administration for 14 days and 28 days,24 h TP in Chinese medicine group and Western medicine group was significantly lower than that of model group(P<0.05),and that of the Chinese medicine group was lower than that of the Western medicine group(P<0.05).The ALB content of model group,Chinese medicine group and Western medicine group was lower than that of normal control group(P<0.01),the ALB content of Chinese medicine group was higher than that of Western medicine group(P<0.05),and the ALB comtent of Western medicine group was higher than that of model group(P<0.05).There were no significant differences in BUN and SCr among all groups(P>0.05).VEGF expression in plasma and kidney tissue of model group,Chinese medicine group and Western medicine group was significantly higher than that of normal control group(P<0.05),VEGF expression in the Chinese medicine group was lower than that in the Western medicine group(P<0.05),VEGF expression in the Western medicine group was lower than that in the model group(P<0.05).Conclusion:Micromeria biflora can relieve proteinu-ria in rats with doxorubicin-induced nephropathy,and its mechanism may be related to the down-regulation of VEGF expression in plasma and renal tissue.
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