Neuroprotective Effect of Eupatilin on Mice with Cerebral Hemorrhage and Influences on Oxidative Stress and Neurocytes Apoptosis
Objective:To explore the neuroprotective effect and mechanism of Eupatilin on intracerebral hemorrhage(ICH)mice.Methods:The ICH model mice were established by autologous blood injection me-thod,and sham operation mice were set at the same time.ICH mice were orally administered 1,3,10 mg/kg Eupatilin for 3 days.Longa score was used to evaluate the neurological deficit.Mice brain tissue were collected,weighted to determine the moisture content,staining to observe neuron changes and Sestrin2 expression,ELISA was used to detect MDA level and SOD,CAT activities,Western blot was used to determine Sestrin2,Bcl-2,Bax,caspase-3,AMPK,p-AMPK,GSK-3β,p-GSK-3β protein expressions.Results:Eupatilin could alleviate nerve defect of ICH mice(P<0.05 or P<0.01),with time-dose dependent;as well decreased brain tissue moisture content(P<0.05 or P<0.01),reduced neuronal apoptosis(P<0.01),increased the number of nissl bodies(P<0.05 or P<0.01),reduced MDA level(P<0.01),increased SOD and CAT activities(P<0.01),and decreased Bax and caspase-3 protein expressions(P<0.05 or P<0.01),and increased the ex-pressions of Sestrin2,Bcl-2,p-AMPK/AMPK,and p-GSK-3β/-GSK-3β protein expressions(P<0.05 or P<0.01)with dose dependent.Conclusion:Eupatilin exerts a neuro-protective effect on ICH mice by inhibiting oxidative stress and neuronal apoptosis.
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