Study on Neuroprotection of Shuji Decoction(舒肌汤)on Levodopa-Induced Dyskinesia in Parkinson Rats
Objective:To observe the neuroprotection effect a of Shuji Decoction(SJD)on dyskinesia in-duced by L-dopa in Parkinson's(PD)rats and explore the mechanism.Methods:SD rats were used to estab-lish PD model by by injecting 6-OHDA,then intraperitoneally injected with L-dopa and benzyl hydrazine to induce dyskinesia,and randomly divided into model group,low dose SJD group(10 g/kg),medium dose SJD group(20 g/kg),and high dose SJD group(40 g/kg),sham operation group(operations were replaced by nor-mal saline)was set up at same time.Intragastric administration continuously for 28 d and evaluated the abnormal involuntary movement(AIM)after medication for1 d,7d,14 d,21 d,28 d.Single leg steps of rats with weight bearing were determined;ELISA was used to detect the levels of brain MDA,GSH,SOD,IL-1 β,TNF-α,IL-6,IL-10,dual fluorescent immunostaining was used to detect CD11b+Iba1+microglias,and Western blot was used to detect tyrosine hydroxylase(TH)protein expression.Results:Compared to sham group,AIM scores was higher in model group,and single leg steps were decreased,the levels of brain MDA,IL-1 β,TNF-α,IL-6 and IL-10 were increased,while the content of GSH and SOD were decreased,CD11b+Iba1+microglia were increased,and the expression of TH protein decreased(all P＜0.01).Compared to model group,AIMs scores of rats in each SJD group were decreased after 7 days of medication;single leg steps of rats in each SJD group were increased,levels of brain MDA,IL-1 β,TNF-α,IL-6 and IL-10 were decreased,CD11b+Iba1+microglia were reduced,and expression of TH protein were increased(P＜0.05);and levels of GSH,SOD of rats in medium,high SJD groups were increased(P＜0.05).Conclusion:SJD can improve the symp-toms of PD dyskinesia rats induced by L-dopa,and its mechanism may be related to inhibiting the activation of microglia to reduce the release of inflammatory mediators,reduce oxidation stress damage,and promoting the ex-pression of TH protein.
万方数据
维普
