Effect of Zhuyang Shuxin Prescription(助阳舒心方)onBDNF in Hippocampus of Depressed Rats
Objective:To observe the effect of Zhuyang Shuxin Prescription on the behavior of depressed rats,and to explore its anti-depression mechanism by detecting the BDNF level in hippocampus.Methods:50 SD rats were randomly divided into normal group,model group,Zhuyang Shuxin Prescription low,moddle,high dose(2、4、8 g/kg)groups with each10 rats.CUMS and solitary culture were used to induce depression model for 21 days.The change of rats'body weight were recorded,open field test and sugar favouritism test were applied to observe rats'behavioral changes.The levels of BDNF in hippocampus were measured by real-time PCR and IHC.Results:Compared with the normal control group,the growth rate of body weight in the model group was decreased,rats stayed in the central grid for longer time,the number of rats'vertical movements was decreased,and the percentage of sugar water preference was decreased.Both IHC and real-time PCR showed that the level of BDNF in the hippocampus was decreased;Compared with the model group,the growth rate of body weight of the three dose Zhuyang Shuxin Prescription groups was increased,the desire to explore the surrounding environ-ment was enhanced,the percentage of preference for sugar water was increased,and the BDNF level was in-creased.Conclusion:The improvement of symptoms of chronic unpredictable depressed model rats by Zhuyang Shuxin Prescription may be related to the increase of BDNF level in the hippocampus.
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