Study on Effects of Tiansha Mixture(天沙合剂)on Squaporins Expressions of Rats with Skin Xerosis Caused by EGFRIs
Objective:To explore the mechanism of Tiansha Mixture on skin xerosis caused by EGFRIs by regulating aquaporins(AQPs).Methods:36 rats were divided into six groups:normal control,EGFRIs model,positive control,and low,medium,and high dose of Tiansha Mixture groups.All groups except for normal con-trol group rats were Ig Gefitinib for 28 days to build EGFRIs-induced skin dryness model,vitamin E and Three dose of Tiansha Mixture groups were given the corresponding medicine(vitamin E13.5 mg/kg and Tiansha Mix-ture 8、4、2 mg/kg)for 14 days from the 15 th day Ig Gefitinib,normal control and EGFRIs model were given the same volume saline.Trans epidermal water loss(TEWL),HE staining were used to assess skin pathological damage;AQP1,AQP3,and AQP5,expressions of skin tissue were measured by Western blot.Results:Rats'skin rash of rats in vitamin E and high,medium dose Tiansha Mixture groups were significantly improved,TEWL level was reduced.Skin pathological damage of rats in vitamin E and three dose Tiansha Mixture groups were alle-viated,AQP1,AQP3,AQP5 expressions of rats'skin tissue were increased.Conclusion:Tiansha Mixture can re-duce EGFRIs-induced skin xerosis,improve skin tissue pathological injury,raising AQPs expressions was one of the mechanism.
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