Experimental Study on Protective Effects of Huangqi-Sanzhuyu(Astragalus Membranaceus-Cornus Officinalis)on High Glucose-Induced Podocyte Injury by Inhibiting JAK2/STAT3 Signal Pathway
Objective:To explore the improvement effect and mechanism of Astragalus membranaceus-Cornus officinalis(A-C)on podocyte injury induced by high glucose.Methods:CCK8 method was used to detect the activity of podocyte cells(MPC-5)cultured by A-C(0,175,350,700,1400,2800 mg/L).High glucose(30 mmol/L glucose)was used to induce MPC-5 cell damage,350,700,1400 mg/LA-C to intervene.ELISA was used to detect the levels of IL-6 and IL-1β of MPC-5,flow cytometry was used to de-tect apoptosis of;immunofluorescence staining was used to detect the expression of nephrin,podocin;qRT-PCR was used to detect the gene expression of nephrin,podocin,desmin,WT-1;Western blot was used to de-tect the phosphorylation of JAK2/STAT3 signal pathway.Results:2800 mg/L A-C significantly inhibited MPC-5 activity(P＜0.01).The induction of high glucose increased levels of IL-6,IL-1β and apoptosis of MPC-5,reduced expression of nephrin,podocin,WT-1,increased desmin expression and phosphorylation of JAK2/STAT3 signal pathway(P＜0.01).350,700,1400 mg/L A-C inhibited levels of IL-6,IL-1β and apoptosis of MPC-5 induced by high glucose,also increased expression of nephrin,podocin,WT-1,de-creased desmin expression and phosphorylation of JAK2/STAT3 signal pathway(P＜0.05).Conslusion:A-C alleviate inflammation and apoptosis injury of podocyte induced by high glucose,and its mechanism may be relat-ed to inhibiting JAK2/STAT3 pathway phosphorylation.
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