Study on Effects of Shuji Decoction(舒肌汤)on Levodopa-Induced Parkinson's Disease Muscle Rigidity Rat and Striatal Tissue TH,DA Contents and FosB,p-ERK Expressions
Objective:To observe the effects of Shuji Decoction on Levodopa(L-DOPA)induced Parkin-son's disease(PD)muscle rigidity model rats,and explored its mechanism of action.Methods:L-DOPA in-duced the establishment of PD muscle rigidity rat model,Models were randomly divided into model group(equal volume of saline),Shuji Decoction low,medium and high dose groups(1.0,2.0,4.0 g/kg),and a sham-ope-rated group(equal volume of saline)was set up as the control,6 rats in each group,1 time a day continuous administration for 28 d.After completion of drug administration,behavioral performance was detected by rotation count test,rollover reflex test,and electromyography.The expression level of tyrosine hydroxylase(TH)in brain tissue were measured by immunohistochemistry.Dopamine(DA)and TH levels in brain striatum were measured by ELISA.FosB,p-ERK protein expression levels in brain striatum were measured by Western blot.Results:Compared with the sham-operated group,the number of rotations,roll-over reflex time and score,muscle action potential wave amplitude and latency time were significantly higher or longer(P＜0.01)and MCV was significantly lower(P＜0.01)in the model group;compared with the model group,the number of rotations,roll-over reflex time and score,muscle action potential wave amplitude and latency time were significantly lower of shorter(P＜0.05)and MCV was significantly higher(P＜0.01)in Shuji Decoction groups.Immunohistochemistry results showed that Shuji Decoction could up-regulate the expression level of TH.ELISA results showed that Shuji Decoction could up-regulate the contents of DA and TH in striatum.Western blot results showed that Shuji Decoction could down-regulate the expression levels of FosB and p-ERK protein.Conclusion:Shuji Decoction showed significant efficacy in Parkinson's disease muscle rigidity model rats,and its mechanism of action may be related to the up-regulation of DA and TH contents and down-regulation of FosB and p-ERK protein expressions.
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