Analysis of Composition of Volatile Oil of Qiai(Artemisia argyi)by GC-MS and Study on Its Antibacterial Activity
Objective:To analyze of the main chemical constituents of volatile oil from Artemisia argyi,and investigate the antibacterial activity of volatile oil.Methods:Semi-bionic extraction—steam distillation method was used to extract volatile oil from Artemisia argyi.Gas chromatography-mass spectrometry(GC-MS)was app-lied for the identification of chemical constituents in volatile oil from Artemisia argyi and the relative percentage of each component was calculated by area normalization.The antibacterial effect of the volatile oil was investigated by the plate coating method and the double gradient liquid dilution method.Results:50 kinds of chemical com-ponents were detected in volatile oil from Artemisia argyi,and 20 kinds of compounds were identified,accounting for 95.95％of total volatile oil.The chemical constituents were mainly including artemisol(55.49),3,3,6-Trimethyl-1,4-heptadien-6-ol(15.37％),Eucalyptol(11.87％)and 4-methyl-1-(1-methylethyl)-3-Cyclohexen-1-ol(3.50％),mostly composed of al-cohols.The volatile oil from Artemisia argyi had significant antibacterial activity against Staphylococcus aureus,Pseudomonas aeruginosa,Salmonella,Bacillus subtilis,Bacillus licheniformis,Candida albicans,Aspergillus ni-ger and Aspergillus flavus.Conclusion:The volatile oil from Artemisia argyi was mainly composed of alcohols and also has a good antibacterial activity.
volatile oil from Artemisia argyisemi-bionic extraction-steam distillation methodGC-MSchemical constituentsantibacterial activity
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