Regulation Effect of Bushen Huoxue Formula(补肾活血方)on Osteogenesis and Angiogenesis Coupling in Ovariectomized Osteoporosis Mice
Objective:To explore the effect of Bushen Huoxue Formula on bone mass and bone metabolism in ovariectomized osteoporosis mice by regulating osteogenesis and angiogenesis Coupling.Methods:40 female ICR mice were randomly divided into sham group(10 mice)and ovariectomy group(30 mice)by random num-ber table method.The mice in the ovariectomized group were routinely raised for 8 weeks after undergoing ovari-ectomy surgery.3 mice were randomly selected to confirm successful osteoporosis modeling.Then,they were ran-domly divided into a model group and a Chinese medicine group,10 mice in each group.The sham operation group and model group were gavaged with equivalent saline.the Chinese medicine group were gavaged with Bushen Huoxue Formula(8.6 mg/kg).After 8 weeks of feeding,the samples were collected.Micro CT,TRAP staining,immunofluorescence staining(EMCN,CD31),and bone metabolism index were detected and analy-sised.Results:Micro-CT showed that the sham group was the most bone trabeculae,bone trabeculae of the model group was decrease,while that of the Chinese medicine group was increased compared to the model group.The quantitative analysis results showed that the bone volume fraction(BV/TV),number of trabeculae(Tb.N)and thickness of trabeculae(Tb.Th)in the sham group were higher than those in the model group and the Chinese medicine group,while the BV/TV,Tb.N,and Tb.Th in the Chinese medicine group were higher than those in the model group(P＜0.05).Bone tissue TRAP staining and quantitative analysis showed that the number of TRAP positive expressions in the model group was higher than that in the sham group and the Chinese medicine group,while the number of TRAP positive expressions in the sham group was lower than that in the Chinese medicine group(P＜0.05).The proportion of epiphyseal H-vessels in the model group was signifi-cantly reduced compared to the sham group,while the proportion of epiphyseal H-vessels in the Chinese medi-cine group was increased compared to the model group,but was lower than that of the model group(P＜0.05).Compared with the sham group,the PINP level in the model group was decreased and the TRACP level was in-creased(P＜0.05).The PINP level in the Chinese medicine group was increased compared to the model group,while TRACP was decreased,with a statistically significant difference(P＜0.05).Conclusion:Bushen Huoxue Formula can promotes osteogenesis and angiogenesis,thereby increasing bone formation,reducing bone resorp-tion,and increasing bone mass in ovariectomized osteoporosis mice.
Bushen Huoxue Formulaosteoporosiscastrationbone metabolismosteogenesis and angiogene-sis couplingmouse
万方数据
维普
