Effects of Qinbai Qingfei Concentrated Pill(芩百清肺浓缩丸)on NLRP3/Caspase-1/IL-1β Signal Pathway in Rats with Post-Infection Cough
Objective:To observe the effects of Qinbai Qingfei Concentrated Pills(Qinbai)on NLRP3/Caspase-1/IL-1 β signaling pathway in rats with post-infection cough(PIC).Methods:40 healthy male SD rats were randomly divided into normal group,model group,positive control group(Asmeton)and Qinbai Qingfei Concentrated Pills group(Qinbai),with 10 rats in each group.The PIC model was constructed by smoking combined with LPS nasal drip and aerosol inhalation of capsaicin.After modeling(day 18),intragastric adminis-tration was started.The Qinbai group was given Qinbai Qingfei Concentrated Pills solution 1.65 g/kg,and the positive control group was given Asmeton 25.11 mg/kg,each group was administered once daily for 10 days.Af-ter the last intervention,the level of IL-1 βin BALF was detected by ELISA;the protein expressions of NLRP3 and Caspase-1 in lung tissues were detected by Western blot.Results:Compared with normal group,the levels of IL-1 βin BALF and the protein expressions of NLRP3 and Caspase-1 in lung tissues in the model group were significantly increased(P＜0.05).Compared with model group,those in Qinbai group and positive control group were significantly decreased(P＜0.05).Moreover,the levels of IL-1 βand the levels of NLRP3 protein ex-pression in lung tissue in Qinbai group were significantly lower than those in control group(P＜0.05).Conclu-sion:Qinbai Qingfei concentrated pill can significantly reduce airway inflammation and airway hyperreactivity in rats with PIC,and its mechanism may be related to inhibiting NLRP3/Caspase-1/IL-1β pathway.
万方数据
维普
