Inhibition Effects of Berberine on Bone Loss in Ovariectomized Mice through VDR/GPX4 Pathway
Objective:To study the inhibition effect of Berberine on bone loss in ovariectomized(OVX)mice and its possible mechanism.Methods:40 mice were randomly divided into four groups:sham group,OVX group(model group),OVX+Berberine group(50 mg/kg)(Berberine group),OVX+β-estradiol group(100μg/kg)(E2 group).Each group was given the corresponding medicine for 12 weeks.Western blot was used to detect the protein expressions of bone resorption related factors RANKL and c-Fos.RT-PCR was used to detect the gene expressions of TRAP,cathepsin K and MMP-9,and the regulation of VDR/GPX4 pathway.ELISA was used to detect the MDA level of bone tissue in each group.Results:Compared with the sham group,the expressions of RANKL,c-Fos protein and TRAP,cathepsin K,MMP-9 mRNA,MDA level in the model group were significantly increased(P＜0.01),expressions of VDR,GPX4 protein were decreased(P＜0.01),and GPX4 mRNA level was decreased(P＜0.01).Compared with model group,the the expressions of RANKL,c-Fos protein and TRAP,cathepsin K,MMP-9 mRNA,MDA level in Berberine group and E2 group were significantly decreased(P＜0.01),while the expressions of VDR,GPX4 protein were increased(P＜0.01),and the mRNA level of GPX4 was up-regulated(P＜0.01).Conclusion:Berberine can reduce bone loss in ovariectomized mice by activating VDR/GPX4 pathway.
万方数据
维普
