Exploration on Mechanisms of Gancao Xiexin Decoction in the Treatment of Behcet's Disease Based on Network Pharmacology
Objective:To analyze the potential active ingredients,possible targets and molecular mechanisms of Gancao xiexin decoction in the treatment of Behcet's disease(BD)based on network pharmacology.Methods:According to the composition of Gancao xiexin decoction,the active ingredients and their targets were screened on the database and analysis platform of traditional Chinese medicine systematic pharmacology(TCMSP).The database of GeneCard,OMIM,PharmGkb and DrugBank were used to collect BD-related targets with"Behcet's disease"as the search term.Meanwhile,the data of drug targets and disease targets were compared to obtain intersection targets.Cytoscape 3.8.2 software was used to perform visual network analysis of drug-disease targets.Drug-disease target results were subjected to enrichment analysis of Gene Ontology(GO),whole-genome and metabolic pathway analysis of Kyoto Encyclopedia of Genes and Genomes(KEGG).Results:There were 174 active ingredients in TCSMP database,including quercetin,kaempferol,wogonin,baicalein,β-carotene and acacetin.There were 247 Gancao xiexin decoction potential targets,1006 BD-related targets,and 60 intersection targets of active ingredients of Gancao xiexin decoction and BD-related target genes.The core targets were HMOX1,IFNG,CAT,EGF,VEGFA,PTGS2,CXCL8,TP53,IL1B,STAT3,SERPINE1,MPO,CCL2,SELE,ICAM1,VCAM1,MMP9,CASP3,NFKBIA,IL1A.A total of 1697 items were obtained by GO enrichment analysis,which mainly involved the response to lipopolysaccharide,molecular response to the bacterial origin and exogenous apoptotic signaling pathway and other biological processes.KEGG enrichment analysis revealed 114 signaling pathways,including AGE-RAGE signaling pathway in diabetic complications,fluid shear stress and atherosclerosis and tumor necrosis factor signaling pathway.Conclusion:Gancao xiexin decoction can treat BD through multi-target and multi-pathway,which provides bioinformatics basis for further revealing its mechanism.
Gancao xiexin gecoctionBehcet's diseasenetwork pharmacologyactive ingredienttarget of action
万方数据
维普
