Exposed to Electromagnetic Radiation Based on Full-Length 16S rRNA Sequencing
Objective:To describe and compare the distribution characteristics of oral microbiome between workers exposed to electromagnetic radiation and from normal environment,so as to provide a potential screening method and rationale for early prevention of the damage caused by electromagnetic radiation.Methods:A total of 130 staff from two units were randomly selected from October to December in 2023 and divided into two groups according to their working environment,i.e.,electromagnetic radiation group(JMS group,n=65)and normal group(BJ group,n=65).Saliva samples were collected to extract DNA,followed by 16S rRNA sequencing by high-throughput gene sequencing platform,and the oral microbiome was characterized and compared between the two groups.Results:Beta diversity analysis showed that there was statistically significant difference in the structure of oral microbiome between JMS and BJ groups(P<0.01).In terms of specific composition of oral microbiome,at phylum level,the relative abundance of Bacteroidetes and Fusobacteria were significantly different between the two groups(P<0.05);At class level,the relative abundance of Bacilli,Fusobacteria and Clostridia were highly significantly different between the two groups(P<0.001),and the relative abundance of Actinomycetia,Coriobacteriia,Tissierella and Bacterioidia were significantly different(P<0.05).The results of LEfSe analysis showed that the structure of microbiome was unique in the JMS group,with significant enrichment of 11 species,including Clostridia,Neisseria subflava A,Cloacibacterium,Cloacibacterium normanense,Oribacterium parvum,Fusobacteria(phylum),Fusobacteriia(class),Fusobacteriales,Oribacterium asaccharolyticum,Filifactor alocis,and Fusobacteriaceae.In addition,through the function prediction by Tax4Fun2,we found that the oral microbiome in the JMS group were enriched in various pathways including immune system,anti-tumor drug resistance,and energy metabolism,and the enrichment degree was higher than that in the BJ group.The enrichment degree through carbohydrate metabolism pathway and membrane transport pathway in the BJ group was higher than that in the JMS group.Conclusion:The characteristics of oral microbiome and its metabolism are different between the populations working in the normal environment and with exposure to the electromagnetic radiation,as demonstrated at multiple levels,suggesting the possible impact of electromagnetic radiation working environment on the distribution characteristics of oral microbiome,which needs further confirmation and exploration.
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