Exploration on the Mechanism of Danzhi Jiangtang Capsules in the Treatment of Diabetic Nephropathy Based on Network Pharmacology and Molecular Docking Technology
Objective To analyze the molecular mechanism of Danzhi Jiangtang Capsules in the treatment of diabetic nephropathy(DN)by network pharmacology and molecular docking technology.Methods The active components and related targets of Danzhi Jiangtang Capsules were obtained through the TCMSP database and literature search,and UniProt database was used to find the gene name corresponding to the mark;DN-related targets were screened from GeneCards,OMIM,and TTD databases.Venny 2.1.0 was used to construct Venn diagrams to obtain common targets.Then,the common targets were uploaded to the STRING database to build a protein interaction network,and the core targets were mined using CytoHubba.The Metascape database was used for GO functional enrichment analysis and KEGG pathway enrichment analysis for potential targets.Finally,AutoDock Vina software was used to do molecular docking between the core components and the core targets.Results Totally 26 active components of Danzhi Jiangtang Capsules were obtained,corresponding to 267 targets.Its core components included quercetin,luteolin,kaempferol,etc.853 DN related targets and 109 intersecting targets with the active component targets of Danzhi Jiangtang Capsules,with core targets including EGFR,TP53,IL6,etc.GO and KEGG analysis showed that Danzhi Jiangtang Capsules may play a therapeutic role by regulating AGE-RAGE signaling pathway in diabetic complications,insulin resistance,relaxin signaling pathway,and participation in enzyme binding,apoptosis,proliferation,and migration,etc.Molecular docking results showed that the core components of Danzhi Jiangtang Capsules had structurally stable to the core targets,and the binding activity was good.Among them,the binding energy of luteolin and EGFR was the lowest.Conclusion Danzhi Jiangtang Capsules can exert therapeutic effects on DN through multiple pathways,regulating the molecular network centered on key target groups such as TNF,STAT3,VGEFA,and IL6,and regulating AGE-RAGE,insulin resistance and relaxin signaling pathway to inhibit oxidative stress response and renal tissue fibrosis.
国家科技期刊平台
NSTL
万方数据
维普
