Exploration on the Mechanism of Jianpi Yishen Decoction in the Treatment of Diabetic Nephropathy Based on Network Pharmacology and Molecular Docking
Objective To explore the molecular mechanism of Jianpi Yishen Decoction in the treatment of diabetic nephropathy(DN)through network pharmacology and molecular docking.Methods The main active components and targets of Jianpi Yishen Decoction were obtained by searching TCMSP.GeneCards,OMIM,DrugBank,TTD and DisGeNET databases were searched to obtain DN-related targets,and Venny 2.1 was used to obtain the intersection targets of drugs and disease targets.The protein-protein interaction network was constructed by BisoGenet,and the intersection network was extracted to obtain the key target of Jianpi Yishen Decoction in the treatment of DN.Metascape database was used to analyze the GO function and KEGG pathway enrichment of the intersection targets.Cytoscape 3.9.1 software was used to construct the"active component-target-signaling pathway"network,and the main active components and core targets of Jianpi Yishen Decoction in the treatment of DN were explored.AutoDock Vina was used for molecular docking.Results A total of 197 active components,363 corresponding targets and 1 347 DN-related targets were obtained.The core targets of Jianpi Yishen Decoction in the treatment of DN included TUBB,HNRNPK,NCL,PABPC1,DHX9 and so on.GO functional enrichment results showed that biological processes were mainly involved in the response to hormones and the positive regulation of cell migration.Cell components mainly affected membrane raft,vesicle cavity,endoplasmic reticulum cavity,extracellular matrix,etc.Molecular functions included protein kinase binding,RNA polymerase Ⅱ specific DNA binding transcription factor binding,signal receptor activator activity and other protein,enzyme and lipid binding.KEGG pathway enrichment analysis obtained 211 pathways,mainly involving pathways in cancer,AGE-RAGE signaling pathways in diabetic complication,proteoglycans in cancer,chemical carcinogenesis-reactive oxygen species,and HIF-1 signaling pathways.Conclusion Jianpi Yishen Decoction can act on signaling pathways such as pathways in cancer,AGE-RAGE signaling pathways in diabetic complication through multiple targets such as TUBB,HNRNPK,NCL,PABPC1,DHX9,and exert anti-inflammatory and antioxidant effects,regulate metabolic disorders,regulate cell apoptosis and autophagy,and play a therapeutic role in DN.
国家科技期刊平台
NSTL
万方数据
维普
