Exploration on the Mechanism of Guipi Decoction in Treating Immune Thrombocytopenia Based on Network Pharmacology and Molecular Docking
Objective To analyze the mechanism of Guipi Decoction(GPD)in treating immune thrombocytopenia(ITP)based on network pharmacology and molecular docking.Methods TCMSP,TCMIP and BATMAN databases were used to collect the active components and targets of GPD.ITP related targets were obtained by GeneCards,OMIM and TTD.The intersection targets of them and protein protein interaction(PPI)network were obtained by jvenn and STRING online platform.Cytoscape was used to construct and analyze the network diagrams,and GO analysis and KEGG pathway enrichment were performed by DAVID database.Molecular docking was carried out by AutoDock.Results GPD included 175 chemical components and 491 targets.1 133 targets of ITP were identified.Network diagram showed that core targets of GPD in treating ITP were TNF,and the key active components were quercetin and so on.There were 507 Go enrichment items and 109 KEGG pathways.The molecular docking results showed that the core active components and the core targets had good binding activity.Conclusion GPD maybe act on TNF,IL6,IL1B,IL10 and IFNG by quercetin,kaempferol,β-sitosterol,fomononetin,and isorhamnetin to play anti-inflammatory and the immune regulation role through TNF signaling pathway ect.,which can provide a theoretical basis for the mechanism of GPT in treating ITP.
国家科技期刊平台
NSTL
万方数据
维普
