摘要
目的 基于网络药理学方法研究芩蓟凉血合剂治疗便秘的作用机制.方法 通过TCMSP、TCMID数据库获取芩蓟凉血合剂活性成分及对应靶点,再通过GeneCards、TTD、DrugBank、OMIM、DisGeNET数据库获取便秘相关靶点,将共有靶点导入STRING数据库构建蛋白相互作用(PPI)网络,利用Cytoscape3.8.1软件构建芩蓟凉血合剂治疗便秘中药-活性成分-靶点网络,筛选关键成分与核心靶点,并利用Metascape数据库进行GO功能和KEGG通路富集分析.结果 从芩蓟凉血合剂中筛选出槲皮素、山柰酚、木犀草素、β-谷甾醇、豆甾醇共5个关键成分,HSP90AA1、STAT3、MAPK3、TP53、AKT1、MAPK1、RELA、TNF、FOS及IL6共10个核心靶点.KEGG通路富集分析结果显示,芩蓟凉血合剂治疗便秘主要涉及癌症通路、HIF-1信号通路、脂质和动脉粥样硬化等信号通路.结论 芩蓟凉血合剂可能通过HIF-1信号通路、脂质和动脉粥样硬化等信号通路,调控HSP90AA1、STAT3、MAPK3等靶点治疗便秘.
Abstract
Objective To study the mechanism of Qinji Liangxue Mixture in the treatment of constipation based on network pharmacology.Methods The effective components and action targets in Qinji Liangxue Mixture were obtained from TCMSP and TCMID.Constipation-related targets were retrieved through GeneCards,TTD,DrugBank,OMIM,and DisGeNET databases.The STRING database was used to construct the common target protein-protein interaction(PPI)network PPI.Cytoscape 3.8.1 software was used to construct a network map for the treatment of constipation with Qinji Liangxue Mixture of Chinese materia medica-effective components-disease targets,and key components and core targets were screened.GO analysis and KEGG pathway enrichment analysis of target genes were performed using Metascape platform.Results Five key components(quercetin,kaempferol,luteolin,β-sitosterol,and sterol)and 10 core targets(HSP90AA1,STAT3,MAPK3,TP53,AKT1,MAPK1,RELA,TNF,FOS and IL6)were screened from Qinji Liangxue Mixture.KEGG enrichment analysis showed that target-related pathways for the treatment of constipation were mainly involved in pathways in cancer,lipid and atherosclerosis,calcium signaling pathways,and AMPK signaling pathways.Conclusion Qinji Liangxue Mixture may regulate HSP90AA1,STAT3,MAPK3 and other targets to treat constipation through AMPK,lipid,atherosclerosis and other signaling pathways.
基金项目
国家自然科学基金(81873308)
上海市卫生健康委员会中医药科研项目(2022QN011)
上海中医药大学产业发展中心医养结合科创项目(YYKC-2021-01-140)
国家科技期刊平台
NSTL
万方数据