Study on the Mechanism of Shenling Pixi Decoction in Preventing and Treating Chronic Gastritis,Gastric Intraepithelial Neoplasia and Early Gastric Cancer Based on GEO Gene Chip Combined with Network Pharmacology and Molecular Docking Technology
Objective To explore the mechanism of Shenling Pixi Decoction in the prevention and treatment of chronic gastritis,gastric intraepithelial neoplasia and early gastric cancer based on GEO gene chip,network pharmacology and molecular docking technology.Methods A chip GSE130823 containing genes for chronic gastritis,gastric intraepithelial neoplasia and early gastric cancer was downloaded from the GEO database,and R4.1.1 was used to screen for co differentially expressed genes.The TCMSP platform was used to screen the effective chemical components and corresponding target of Shenling Pixi Decoction,and R4.1.1 was used to obtain the intersection of differentially expressed gene and drug target.Cytoscape 3.9.1 software was used to construct a Chinese materia medica-active component-target network,and protein interaction(PPI)network construction and topology analysis were performed on the STRING platform to screen core target.R4.1.1 was used to perform GO function and KEGG pathway enrichment analysis on the intersection targets.Finally,molecular docking verification was performed between the active components of the top ranked drugs and the core target protein structure.Results A total of 1 115 differentially expressed genes were obtained.119 active components and 247 target were selected from the Shenling Pixi Decoction.The main active components included quercetin,stigmasterol,luteolin,etc.22 potential targets for the treatment of CG,GIN and EGC with Shenling Pixi Decoction were obtained after prediction,including CHRM3,AR,ADRB2,DPP4,etc.The GO function and KEGG enrichment analysis results showed that the treatment of CG,GIN,and EGC with Shenling Pixi Decoction mainly involved the regulation of cAMP,IL-17,TNF,ion transmembrane transport protein activity,calcium signaling pathways,and other aspects.The molecular docking results showed that luteolin has strong binding ability with ALB,AR,and DPP4,quercetin has strong binding ability with ALB and DPP4,and stigmasterol has good docking with AR.Conclusion Multiple active components in Shenling Pixi Decoction may regulate target such as ALB,AR,DPP4 and CYP3A4 for treating CG,GIN,and EGC through signaling pathways such as cAMP,IL-17 and TNF.
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