Exploration on the Medication Law and Mechanism of Huoxue Huayu Method in the Treatment of MAFLD Based on Data Mining and Network Pharmacology
Objective To explore the medication law and mechanism of representative drugs of Huoxue Huayu(promoting blood circulation and removing blood stasis)Method in the treatment of metabolic-related fatty liver disease(MAFLD)based on data mining and network pharmacology.Methods Randomized controlled trials(RCTs)of Huoxue Huayu Method for the treatment of MAFLD were retrieved from CNKI,Wanfang Data,VIP and CBM from the establishment of the databases to June 1,2023.The frequency of Chinese materia medica,association rules,and clustering analysis were performed using the ancient and modern medical case cloud platform V2.3.7.The core Chinese materia medica-Salviea Miltiorrhizae Radix et Rhizoma was selected for network pharmacology analysis.The active components and targets were screened based on TCMSP database.The disease targets were obtained by searching GeneCards,OMIM and other databases,and the potential genes of Salviea Miltiorrhizae Radix et Rhizoma in the treatment were predicted.R language 4.0.3,Cytoscape 3.7.1 and STRING database were used for pathway analysis and network diagram construction.AutoDuck Vina 1.1.2 and PyMOL 2.3.0 software were used for molecular docking verification.Results A total of 126 prescriptions were included,involving 182 kinds of Chinese materia medica,and Salviea Miltiorrhizae Radix et Rhizoma was the most frequently used Chinese materia medica.The medicinal properties were mainly warm,slightly cold,cold,neutral and slightly warm.The medicinal tastes were mainly pungent,sweet and bitter.The meridian tropism was mainly liver meridian,spleen meridian,lung meridian and stomach meridian.The core compatibility drugs of Huoxue Quyu Method were Salviae Miltiorrhizae Radix et Rhizoma,Crataegi Fructus,Alismatis Rhizoma,Bupleuri Radix,etc.Four clustering prescriptions were obtained by clustering analysis.Salviae Miltiorrhizae Radix et Rhizoma was selected as the representative drug for network pharmacology analysis.The results suggested that there were 65 bioactive components in it,and it may play a role in the treatment of MAFLD through key targets such as RELA,EGFR,VEGFA and the pathway of lipid and atherosclerosis and PI3K-Akt,and predicted components had good combination with the corresponding target proteins.Conclusion This study summarizes the compatibility of prescriptions for Huoxue Quyu Method in the treatment of MAFLD.The representative drug Salviea Miltiorrhizae Radix et Rhizoma can treat MAFLD through multi-targets and multi-pathways.
Huoxue Huayu(promoting blood circulation and removing blood stasis)MethodSalviea Miltiorrhizae Radix et Rhizomafatty liverdata miningnetwork pharmacology
国家科技期刊平台
NSTL
万方数据
