目的 观察原花青素(PC)对大鼠视网膜缺血再灌注(RIR)损伤后视网膜结构及核转录因子-κB(NF-κB)表达的影响,探讨其作用机制.方法 所有大鼠随机分为5 组.于造模前2 周PC 低、高剂量组分别给予100、300 mg/kg剂量灌胃PC 混悬液,丹参组予丹参颗粒溶液0.09 g/kg 剂量灌胃,正常组及模型组给予等量生理盐水灌胃,均每日1 次.造模后各组仍继续按原方案给药,除正常组外,其余各组按再灌注时间的不同分为缺血再灌注6、24、48、72 h 组.以免疫组织化学方法检测各时点视网膜中NF-κB 的表达,通过透射电镜观察各时点视网膜的超微结构.结果 NF-κB在缺血再灌注6 h 后开始表达,24 h 表达最强,然后逐渐下降.PC 低、高剂量组及丹参组明显低于缺血再灌注模型组同期NF-κB 的表达(P ＜0.01).PC 高剂量组NF-κB 的表达明显受到抑制(P ＜0.01).电镜下可见48 h 模型组视网膜各层细胞超微结构异常程度明显重于6、24 h.模型组神经节细胞层见部分神经节细胞胞质内细胞器溶解、消失,细胞核固缩,异染色质增多,染色质边集;丹参组及PC 低剂量组神经节细胞胞质内细胞器肿胀;PC 高剂量组神经节细胞层结构基本正常.结论 PC 对大鼠RIR 损伤中NF-κB 的表达具有抑制作用,从而对RIR 损伤有保护作用.
Effect of Proanthocyanidins on Retinal Structure and NF-κB Expression on Retinal Ischemia-reperfusion Injury of Rats
Objective To observe the effect of proanthocyanidins (PC) on retinal structure and NF-κB expression of retina ischemia-reperfusion (RIR) rats after injury, and explore its possible protection mechanisms. Methods All rats were randomly divided into five groups. In two weeks before modeling, PC low-, high-dose group were given 100, 300 mg/kg PC suspension, salvia miltiorrhiza group was given salvia miltiorrhiza particle solution 0.09 g/kg, normal group and model group were given normal saline, 1 time/d, and continued after modeling. Except for the normal group, other groups were divided into ischemia-reperfusion 6, 24, 48 and 72 h group. Immunohistochemistry methods were used to test retina NF-κB expression in each period, and ultrastructure of the retina was observed through the transmission electron microscope. Results The NF-κB began to express at 6 h after ischemia-reperfusion, expression was the strongest at 24 h, and then gradually decreased. NF-κB expression of PC low-, high-dose group and salvia miltiorrhiza group were significantly lower than that of the model group in the same period (P<0.01). The NF-κB expression of PC high-dose group was restrained significantly (P<0.01). By electron microscopy observation, 48 h model group ultrastructure abnormalities of retinal each layer cell were more severe than 6, 24 h. In model group, part of the cytoplasm ganglion cell organelles of ganglion cell layer dissolved, disappeared, nucleus pycnosis, heterochromatin increased, chromatin edge set. In salvia miltiorrhiza group and PC low-dose group, the organelles of ganglion cells in the cytoplasm swelling. The ganglion cell layer structure of PC high-dose group was normal. Conclusion PC has an inhibitory effect on NF-κB expression, so as to has protective effect from RIR damage.
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