首页|LiBr辅助球磨预处理提升玉米芯木糖渣酶解转化葡萄糖效率的研究

LiBr辅助球磨预处理提升玉米芯木糖渣酶解转化葡萄糖效率的研究

扫码查看
本研究以玉米芯木糖渣(CCR)为原料,研究了LiBr辅助球磨预处理促进CCR酶解转化葡萄糖的效果.经单因素分析,LiBr辅助球磨预处理CCR较优的工艺条件为:球磨时间6.0h、LiBr添加量50%和CCR固体含量80%;此预处理条件下的CCR无需水洗分离LiBr,在保留木质素的情况下实现了 CCR的高效酶解;在纤维素酶用量为5 FPU/gCCR的情况下,酶解葡萄糖产率可高达95%.分析表征结果显示,LiBr辅助球磨预处理可高效破坏CCR中纤维素的结晶结构,降低了结晶度,使其具有无定形结构,提高了CCR的孔隙率,从而显著促进了CCR的酶水解.
Study on LiBr-assisted Ball Milling Pretreatment for Promoting the Efficiency of Enzymatic Hydrolysis of Corncob Residues to Glucose
In this study,corncob residues(CCR)were used as raw materials to investigate the effects of LiBr-assisted ball milling pre-treatment on enzymatic hydrolysis of CCR to glucose.Based on the monofactor analysis,it was concluded that the suitable conditions of LiBr-assisted ball milling pretreatment of CCR were as follows:ball milling time of 6.0 h,LiBr dosage of 50%,and solid content of CCR of 80%.Under the suitable pretreatment conditions,LiBr did not need water washing to separate,and efficient enzymatic hydrolysis of CCR was realized with lignin retention.At the cellulase dosage of 5 FPU/gCCR,the enzymatic glucose yield of pretreated CCR reached to 95%.The results of analysis and characterizations showed that LiBr-assisted ball milling could efficiently destroy the crystal structure of cellulose in CCR,reduce the crystallinity,make it have an amorphous structure,and increase the porosity of CCR,thus significantly pro-moting the enzymatic hydrolysis of CCR.

corncob residuepretreatmentlithium bromideball millingenzymatic hydrolysisglucose yield

张文璐、李海明、于光、李滨

展开 >

大连工业大学轻工与化学工程学院,辽宁省生物质化学与材料重点实验室,辽宁木质纤维生物质精炼协同创新中心,辽宁大连,116034

中国科学院青岛生物能源与过程研究所,中国科学院生物燃料重点实验室,山东青岛,266101

玉米芯 预处理 溴化锂 球磨 酶解 葡萄糖产率

国家自然科学基金山东能源研究院科研创新基金

U22A20423SEIS202106

2024

中国造纸
中国造纸学会 中国制浆造纸研究院

中国造纸

北大核心
影响因子:0.525
ISSN:0254-508X
年,卷(期):2024.43(1)
  • 22