Study on LiBr-assisted Ball Milling Pretreatment for Promoting the Efficiency of Enzymatic Hydrolysis of Corncob Residues to Glucose
In this study,corncob residues(CCR)were used as raw materials to investigate the effects of LiBr-assisted ball milling pre-treatment on enzymatic hydrolysis of CCR to glucose.Based on the monofactor analysis,it was concluded that the suitable conditions of LiBr-assisted ball milling pretreatment of CCR were as follows:ball milling time of 6.0 h,LiBr dosage of 50%,and solid content of CCR of 80%.Under the suitable pretreatment conditions,LiBr did not need water washing to separate,and efficient enzymatic hydrolysis of CCR was realized with lignin retention.At the cellulase dosage of 5 FPU/gCCR,the enzymatic glucose yield of pretreated CCR reached to 95%.The results of analysis and characterizations showed that LiBr-assisted ball milling could efficiently destroy the crystal structure of cellulose in CCR,reduce the crystallinity,make it have an amorphous structure,and increase the porosity of CCR,thus significantly pro-moting the enzymatic hydrolysis of CCR.