In this study,corncob residues(CCR)were used as raw materials to investigate the effects of LiBr-assisted ball milling pre-treatment on enzymatic hydrolysis of CCR to glucose.Based on the monofactor analysis,it was concluded that the suitable conditions of LiBr-assisted ball milling pretreatment of CCR were as follows:ball milling time of 6.0 h,LiBr dosage of 50％,and solid content of CCR of 80％.Under the suitable pretreatment conditions,LiBr did not need water washing to separate,and efficient enzymatic hydrolysis of CCR was realized with lignin retention.At the cellulase dosage of 5 FPU/gCCR,the enzymatic glucose yield of pretreated CCR reached to 95％.The results of analysis and characterizations showed that LiBr-assisted ball milling could efficiently destroy the crystal structure of cellulose in CCR,reduce the crystallinity,make it have an amorphous structure,and increase the porosity of CCR,thus significantly pro-moting the enzymatic hydrolysis of CCR.
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