This study investigated a calcified anaerobic reactor at a waste paper pulping company,where granular samples were col-lected over a year. Systematic analysis of the physicochemical and biological properties of the granules revealed that calcium carbon-ate accumulation during calcification led to increased Ca2+content,higher ash content,accelerated settling velocity and larger diam-eter. Prolonged exposure to high-calcium wastewater significantly enhanced the relative abundance of hydrogen-producing aceto-gens in the bacterial community,while the abundance of hydrolytic bacteria and acidogens declined. In the archaeal community,the original dominant methanogens Methanobacterium decreased,while archaea adapted to the high-calcium environment showed significant growth,such as Bathyarchaeia,Methanolinea,and Candidatus_Methanofastidiosum. These changes in physicochemical properties and microbial community succession provided critical insights into the response mechanisms of granular sludge under cal-cification stress.
关键词
废纸制浆废水/钙化/颗粒污泥/微生物群落
Key words
waste paper pulping wastewater/calcification/granular sludge/microbial community