摘要
目的 研究除草剂丁草胺对泥鳅(Misgurnus anguillicaudatus)体内重要器官过氧化物酶(POX)、非特异性酯酶(NSE)、碱性磷酸酶(ALP)、酸性磷酸酶(ACP)、琥珀酸脱氢酶(SDH)和腺苷三磷酸酶(ATPase)等6种代谢与免疫相关酶活力的影响.方法 将泥鳅暴露在不同浓度的丁草胺中96 h,解剖取其心脏、肝胰脏、鳃、头肾、胃和肠等6种器官,采用冰冻切片、酶组织化学及光密度分析技术检测酶活力变化.结果 POX在头肾中活性显著较高,其余器官中活性均较低;高浓度丁草胺显著抑制头肾POX活性,但显著促进肝胰脏、胃和肠POX活性.NSE在肝胰脏中活性显著较高,心脏中活性显著较低;高浓度丁草胺对除心脏和胃以外的体内器官NSE活性具有显著抑制作用.ALP在胃中活性显著较高,心脏和头肾中未检测出明显的酶活性;高浓度丁草胺显著抑制胃和肠ALP活性,对肝胰脏和鳃ALP活性无显著影响.ACP在胃和肠中活性显著较高,心脏中活性显著较低;高浓度丁草胺显著抑制肝胰脏、鳃、头肾、胃和肠ACP活性,对心脏ACP活性无显著影响.SDH在心脏中活性显著较高,鳃和头肾中未检测出明显的酶活性;高浓度丁草胺显著抑制心脏、肝胰脏和胃SDH活性,对肠SDH活性无显著影响.ATPase在心脏中活性显著较高,头肾和肠中活性显著较低;高浓度丁草胺显著抑制心脏、肝胰脏和头肾ATPase活性,但对胃和肠ATPase活性具有显著促进作用.结论 丁草胺可能通过抑制心脏SDH和ATPase活性影响泥鳅的血液循环,通过抑制胃和肠ALP和ACP活性降低泥鳅对营养物质的消化和吸收能力,通过抑制肝胰和头肾NSE、ACP和ATPase活性损害泥鳅的解毒与免疫防御功能.
Abstract
Objective To investigate the effects of herbicide butachlor on the activities of six metabolic and immune-related en-zymes,including peroxidase(POX),nonspecific esterase(NSE),alkaline phosphatase(ALP),acid phosphatase(ACP),succinate dehy-drogenase(SDH)and adenosine triphosphatase(ATPase)in the vital organs of Misgurnus anguillicaudatus.Methods The Misgurnus anguillicaudatus was exposed to butachlor at different concentrations for 96 h,then six organs,including heart,hepatopancreas,gill,head-kidney,stomach and intestines,were dissected.The changes of enzyme activity were detected by frozen section,enzyme histo-chemistry and photodensitometry.Results POX activity was significantly higher in the head-kidney and lower in the remaining organs;high concentration of butachlor significantly inhibited the POX activity in head-kidney,but significantly promoted POX activity in hepatopancreas,stomach and intestines.NSE activity was significantly higher in hepatopancreas and lower in heart;high concentration of butachlor had a significant inhibitory effect on NSE activity in organs other than the heart and stomach.ALP activity was significant-ly higher in stomach,but not detectable in heart and head-kidney;high concentration of butachlor significantly inhibited ALP activity in stomach and intestines,but had no significant effect on ALP activity in hepatopancreas and gill.ACP activity was significantly higher in stomach and intestines,but significantly lower in heart;high concentration of butachlor significantly inhibited ACP activity in hepato-pancreas,gill,head-kidney,stomach and intestines,but had no significant effect on ACP activity in heart.SDH activity was significantly higher in heart,but not detectable in gill and head-kidney;high concentration of butachlor significantly inhibited SDH activity in heart,hepatopancreas and stomach,but had no significant effect on SDH activity in intestines.ATPase had significantly higher activity in the heart,but significantly lower activity in the head-kidney and intestines;high concentration of butachlor significantly inhibited ATPase activity in the heart,hepatopancreas,and head-kidney,but had a significant promoting effect on ATPase activity in the stomach and intestines.Conclusion The butachlor might influence the blood circulation of Misgurnus anguillicaudatus by inhibiting the activities of SDH and ATPase in heart,reduce the digestion and absorption of nutrients by inhibiting the activities of ALP and ACP in stomach and intestines,and impair the detoxification and immune defence of Misgurnus anguillicaudatus by inhibiting the activities of NSE,ACP and ATPase in hepatopancreas and head-kidney.
基金项目
安徽省自然科学基金项目(1808085MC82)
安徽省重要生物资源保护与利用重点实验室建设基金()
NETL
NSTL
万方数据